Recombinant Mouse FLT4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2116
Recombinant Mouse FLT4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2116
Catalog No.: BLPSN-2116
Product Overview
| Tag | Fc |
| Host Species | Mouse |
| Accession | P35917 |
| Synonym | AI323512, Chy, Flt-4, VEGFR-3, VEGFR3 |
| Background | Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the mouse FLT4 (P35917-1) extracellular domain (Met 1-Glu 775) was was fused with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Tyr 25 |
| AA Sequence | Met 1-Glu 775 |
| Molecular Weight | The secreted recombinant mouse FLT4/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 992 a.a. and has a predicted molecular mass of 112 kDa. As a result of glycosylation and proteolytic cleavage, rm FLT4/Fc monomer migrates as three bands (150, 85, 65 kDa) corresponding to the full length and the cleaved two polypeptides respectively in SDS-PAGE under reducing conditions. |
| Purity | >92% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | 1. Measured by its ability to bind human VEGF-D in functional ELISA.2. Immobilized human VEGF-C at 10 ug/mL (100 uL/well) can bind mouse VEGFR3-Fc.The EC50 of mouse VEGFR3-Fc is 0.008 ug/mL.3. Measured by its ability to bind with mouse FIGF-His in a functional ELISA. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
