Recombinant Mouse FLRT2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2103
Recombinant Mouse FLRT2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2103
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | Q8BLU0 |
Synonym | FLRT2 |
Background | Fibronectin Leucine-Rich Transmembrane (FLRT) proteins are glycosylated membrane proteins expressed at the cell surface which localise in a homophilic manner to cell-cell contacts expressing the focal adhesion marker vinculin. FLRT1, FLRT2, and FLRT3, the three genes encode putative type I transmembrane proteins, each containing 1 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT family members may function in cell adhesion and/or receptor signalling. Each member of the FLRT family has a distinct, highly regulated expression pattern, as was seen for the NLRR family. FLRT2 is expressed in a subset of the sclerotome, adjacent to the region that forms the syndetome, suggesting that interaction with FGF signalling may be a general property of FLRT proteins. All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. FLRT proteins have a dual role, promoting FGF signalling and modulating homotypic cell adhesion. FLRT2 played critical roles in craniofacial development, and it was also present in the vomero-nasal organ, mandibular primodia, and the posterior aspects of the unfused and fused secondary palatal shelves. |
Description | A DNA sequence encoding the mouse FLRT2 (Q8BLU0) (Met1-Ser539) was expressed with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Cys 36 |
AA Sequence | Met1-Ser539 |
Molecular Weight | The recombinant mouse FLRT2 comprises 515 a.a. and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 68-78 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.When cells are added to coated plates(5ug/mL,100uL/well), approximately 50%-70% will adhere after 1 hour at 37°C. |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |