Name: Recombinant Mouse Ephrin-B1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1892
Availability: InStock

Recombinant Mouse Ephrin-B1 Protein (His Tag)

Catalog No.: BLPSN-1892

Product Overview

Tag	His
Host Species	Mouse
Accession	NP_034240.1
Synonym	Cek5-L, EFL-3, Elk-L, Epl2, Eplg2, LERK-2, Lerk2, Stra1
Background	Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.
Description	A DNA sequence encoding the mouse EFNB1 (NP_034240.1) extracellular domain (Met 1-Ser 229) was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Lys 30
AA Sequence	Met 1-Ser 229
Molecular Weight	The secreted recombinant mouse EFNB1 consists of 211 a.a. and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNB1 is approximately 33-38 kDa due to glycosylation.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to compete with mouse EphrinB1 / Fc for binding to immobilized mouse EphB3 in a functional ELISA assay.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.