Recombinant Mouse Ephrin A5 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1865
Recombinant Mouse Ephrin A5 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1865
Catalog No.: BLPSN-1865
Product Overview
| Tag | Fc |
| Host Species | Mouse |
| Accession | O08543 |
| Synonym | AL-1, AV158822, EFL-5, Ephrin-A5, Epl7, LERK-7, RAGS |
| Background | Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion. |
| Description | A DNA sequence encoding the mouse EFNA5 isoform long (O08543-1) without the pro peptide (Met 1-Asn 203) was as fused with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gln 21 |
| AA Sequence | Met 1-Asn 203 |
| Molecular Weight | The recombinant mouse EFNA5/Fc is a disulfide-linked homodimer. The reduced monomer consists of 424 a.a. and has a predicted molecular mass of 48.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNA5/Fc monomer is approximately 52 kDa due to glycosylation. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized Mouse EPHA4 at 2 ug/ml (100 ul/well) can bind mouse EFNA5-Fc with a linear ranger of 1.28-32 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
