Recombinant Mouse EphA3 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1803
Recombinant Mouse EphA3 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1803
Catalog No.: BLPSN-1803
Product Overview
| Tag | Fc |
| Host Species | Mouse |
| Accession | NP_034270.1 |
| Synonym | AW492086, Cek4, End3, ETK1, Hek, Hek4, Mek4, Tyro4 |
| Background | EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the extracellular domain of mouse EPHA3 (NP_034270.1)(Met1-His541) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Glu 21 |
| AA Sequence | Met1-His541 |
| Molecular Weight | The recombinant mouse EPHA3/Fc comprises 762 a.a. and has a predicted molecular mass of 85.7 kDa. The apparent molecular mass of the protein is approximately 96 kDa in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA.Immobilized mouse EFNA5-His at 10 ug/ml (100 ul/well) can bind mouse EPHA3-Fc, The EC50 of rat mouse EPHA3-Fc is 12.3-28.9 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
