Name: Recombinant Mouse Dectin-2 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1592
Availability: InStock

Recombinant Mouse Dectin-2 Protein (His Tag)

Catalog No.: BLPSN-1592

Product Overview

Tag	His
Host Species	Mouse
Accession	NP_064385.1
Synonym	Clec4n, Clecsf10, Nkcl
Background	C-type lectin domain family 4 member N (CLEC4N), also known as Dectin-2, is a C-type lectin expressed by dendritic cells (DCs) and macrophages. Members of the C-type lectin domain (CTLD) superfamily are metazoan proteins functionally important in glycoprotein metabolism, mechanisms of multicellular integration and immunity. They share a common fold and are involved in a variety of functions, such as generalized defense mechanisms against foreign agents, discrimination between healthy and pathogen-infected cells, and endocytosis and blood coagulation. Genome-level studies on human, elegans and melanogaster demonstrated almost complete divergence among invertebrate and mammalian families of CTLD-containing proteins (CTLDcps). The vertebrate CTLDcp family was essentially formed early in vertebrate evolution and is completely different from the invertebrate families. The composition of the CTLDcp superfamily in fish and mammals suggests that large scale duplication events played an important role in the evolution of vertebrates. Dectin-2 is important in host defense against C. albicans by inducing Th17 cell differentiation. Dectin-2 constitutes a major fungal pattern recognition receptor (PRR) that can couple to the Syk-CARD9 innate signaling pathway to activate DCs and regulate adaptive immune responses to fungal infection.
Description	A DNA sequence encoding the mouse CLEC4N (NP_064385.1) extracellular domain (Ile 44-Leu 209) was expressed, with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Ile 44
AA Sequence	Ile 44-Leu 209
Molecular Weight	The recombinant mouse CLEC4N comprises 177 a.a. with a predicted molecular mass of 20.9 kDa. As a result of glycosylation, it migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to agglutinate human red blood cells. Mouse CLEC4N at 25 ug/ml could agglutinate 1 % HRBC.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.