Recombinant Mouse Cyclin E Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1521
Recombinant Mouse Cyclin E Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1521
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&GST |
Host Species | Mouse |
Accession | AAH62152.1 |
Synonym | AW538188, CycE1 |
Background | Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells. |
Description | A DNA sequence encoding the mouse CCNE1 (AAH62152.1) (Met1-Glu408) was expressed with the N-terminal His-tagged GST tag at the N-terminus. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | Met |
AA Sequence | Met1-Glu408 |
Molecular Weight | The recombinant mouse CCNE1/GST chimera consists of 645 a.a. and has a calculated molecular mass of 74.8 kDa. The recombinant protein migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized mouse CCNE1 at 10 ug/ml (100 ul/well) can bind biotinylated human CDK4, The EC50 of biotinylated human CDK4 is 48.0-114.0 ng/ml. |
Formulation | Lyophilized from sterile 50mM Tris, 100mM Nacl, 3mM DTT, 0.5mM GSH, 10% gly, pH 8.0.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |