Recombinant Mouse CRABP2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1414
Recombinant Mouse CRABP2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1414
Catalog No.: BLPSN-1414
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | P22935 |
| Synonym | AI893628, Crabp-2, CrabpII |
| Background | Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to thecalycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema. |
| Description | A DNA sequence encoding the mouse CRABP2 (P22935) (Pro 2-Glu 138) was expressed, with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Pro 2-Glu 138 |
| Molecular Weight | The recombinant mouse CRABP2 consisting of 148 a.a. and has a calculated molecular mass of 17.1 kDa. rm CRABP2 migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >96% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
