Recombinant Mouse CEL Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1217
Recombinant Mouse CEL Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1217
Catalog No.: BLPSN-1217
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | NP_034015.1 |
| Synonym | 1810036E18Rik, BAL |
| Background | CEL-maturity onset diabetes of the young (MODY), diabetes with pancreatic lipomatosis and exocrine dysfunction, is due to dominant frameshift mutations in the acinar cell carboxyl ester lipase gene (CEL). Bile-salt activated carboxylic ester lipase (CEL) is a major triglyceride, cholesterol ester and vitamin ester hydrolytic enzyme contained within pancreatic and lactating mammary gland secretions. Carboxyl ester lipase is a digestive pancreatic enzyme encoded by the CEL gene. Mutations in CEL cause maturity-onset diabetes of the young as well as pancreatic exocrine dysfunction. The enzyme carboxyl ester lipase (CEL), also known as bile salt-dependent or -stimulated lipase (BSDL, BSSL), hydrolyzes dietary fat, cholesteryl esters and fat-soluble vitamins in the duodenum. CEL is mainly expressed in pancreatic acinar cells and lactating mammary glands. The human CEL gene resides on chromosome 9q34.3 and contains a variable number of tandem repeats (VNTR) region that encodes a mucin-like protein tail. |
| Description | A DNA sequence encoding the mouse CEL (NP_034015.1) (Met1-Leu534) was expressed with a C-terminal His tag. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Ala 21 |
| AA Sequence | Met1-Leu534 |
| Molecular Weight | The recombinant mouse CEL comprises 525 a.a. and has a predicted molecular mass of 58.2 kDa. The apparent molecular mass of the protein is approximately 133.9 and 59.1 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | (27.3+65.4)% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
