Recombinant Mouse Catalase (CAT) Protein (His&myc)

Name: Recombinant Mouse Catalase (CAT) Protein (His&myc)
Brand: Beta LifeScience
SKU: BLC-11016P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Catalase (CAT) Protein (His&myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P24270
Target Symbol	CAT
Synonyms	Cat; Cas-1; Cas1Catalase; EC 1.11.1.6
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-myc
Target Protein Sequence	SDSRDPASDQMKQWKEQRASQRPDVLTTGGGNPIGDKLNIMTAGSRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITRYSKAKVFEHIGKRTPIAVRFSTVTGESGSADTVRDPRGFAVKFYTEDGNWDLVGNNTPIFFIRDAILFPSFIHSQKRNPQTHLKDPDMVWDFWSLRPESLHQVSFLFSDRGIPDGHRHMNGYGSHTFKLVNADGEAVYCKFHYKTDQGIKNLPVGEAGRLAQEDPDYGLRDLFNAIANGNYPSWTFYIQVMTFKEAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNKNPVNYFAEVEQMAFDPSNMPPGIEPSPDKMLQGRLFAYPDTHRHRLGPNYLQIPVNCPYRARVANYQRDGPMCMHDNQGGAPNYYPNSFSAPEQQRSALEHSVQCAVDVKRFNSANEDNVTQVRTFYTKVLNEEERKRLCENIAGHLKDAQLFIQKKAVKNFTDVHPDYGARIQALLDKYNAEKPKNAIHTYTQAGSHMAAKGKANL
Expression Range	2-527aa
Protein Length	Full Length of Mature Protein
Mol. Weight	64.7
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
Subcellular Location	Peroxisome.
Protein Families	Catalase family
Database References	KEGG: mmu:12359 STRING: 10090.ENSMUSP00000028610 UniGene: PMID: 29402993 Catalase overexpression modulates metabolic parameters in a new 'stress-less' leptin-deficient mouse model. PMID: 28645653 homocysteine reacts with native catalase and/or redox-active transition metal ions to generate thiyl radicals that mediate compound II formation, a temporarily inactive state of the enzyme. PMID: 27848965 Catalase ameliorates diabetes-induced cardiomyopathy through reduced RelA-mediated transcription of BECN1. PMID: 28643395 Mitochondrial catalase induces neoplastic cell transformation through nucleolin-dependent Cox-2 mRNA stabilization. PMID: 29097213 Moderate MnSOD and/or catalase overexpression in desmin-null hearts leads to a marked decrease in intracellular reactive oxygen species, ameliorates mitochondrial and other ultrastructural defects, minimizes myocardial degeneration and leads to a significant improvement of cardiac function. PMID: 28629836 Mitochondrial catalase overexpressed transgenic mice are protected against lung fibrosis in part via preventing alveolar epithelial cell mitochondrial DNA damage. PMID: 27840320 Mice devoid of catalase develop an obese, pre-diabetic phenotype and provide compelling evidence for catalase (or its products) being integral in metabolic regulation. PMID: 27939935 Data indicate that alpha-syntrophin plays an important role in the regulation of oxidative stress from endogenously generated reactive oxygen species (ROS) during myoblast differentiation by modulating the protein stability of catalase. PMID: 28485891 Overexpression of catalase reduced the concentrations of microsomal benzo(a)pyrene phenols and diols/diones by ~45 and 95%, respectively. PMID: 27607467 Results indicate that mitochondrial catalase likely functions to preclude the formation of high levels of H2O2 without perturbing redox-dependent signaling. PMID: 27614223 Age strongly interacts with catalase targeted to the mitochondria, consistent with antagonistic pleiotropy in the reverse of the typical direction. PMID: 27061426 data suggest that catalase is required not only to scavenge reactive oxygen species, but also to protect DNA from oxidative damage during meiotic maturation in mouse oocytes. PMID: 27160095 These results indicate that CAT expression is significantly decreased in the hippocampi of aged animals and decreased CAT expression may be closely associated with aging. PMID: 27221506 catalase protects mouse hearts against diabetic cardiomyopathy, partially by suppressing NF-kappaB-dependent inflammatory responses and associated protein nitration. PMID: 26456065 Hypoxia-generated red blood cells have low catalase and are preferentially destroyed. PMID: 26017143 The study shows how maturation of active catalase can be influenced by nitric oxide, S-nitrosylated GAPDH, and thioredoxin-1, and how maturation may become compromised in inflammatory conditions such as asthma. PMID: 25659933 Data suggest that embryonic catalase is a determinant of risk for EtOH embryopathies. PMID: 26074427 exposure to either 14- or 28-day chronic stress resulted in a depressive-like syndrome, behavioural invigoration and aggression, and decreased activity of two major brain peroxidation enzymes, superoxide dismutase and catalase. PMID: 24786329 The antioxidant catalase counteracts against high fat diet-induced cardiac geometric and functional anomalies possibly via an IKKbeta-AMPK-dependent restoration of myocardial autophagy. PMID: 24993069 The SirT1 regulates the expression of several antioxidant genes in bovine aortic endothelial cells, including Mn superoxide dismutase, catalase, peroxiredoxins 3 and 5, thioredoxin 2, thioredoxin reductase 2, and uncoupling protein 2. PMID: 23461683 The levels of protein carbonyls, reactive oxygen species, and catalase in the cerebral hemispheres of young and aged mice, were evaluated. PMID: 24338129 The expression levels of catalase and SOD2 are decreased in astrocytes when SIRT1 is inhibited. PMID: 24565075 Data indicate that transgenic mice over-expressing human SOD1 or catalase were protected from loss of plasma membrane integrity (LPMI) at early but not late periods of reperfusion. PMID: 23911407 Data indicate no significant difference in CAT and CuZnSOD gene expression, but enzymatic activity of CuZnSOD and MnSOD significantly increased after sleep deprivation. PMID: 23360182 Up-regulation of catalase caused by high-fat diet is designed to protect heart mitochondria from oxidative damage while not perturbing H2O2-mediated signaling. PMID: 23204527 Antioxidant enzyme, catalase, reverses DNA damage response and cellular transdifferentiation in aortic valve sclerosis. PMID: 23241403 Myocardial catalase expression protects against endotoxemic myocardial dysfunction in mice. PMID: 22902401 catalase overexpression in renal proximal tubular cells ameliorated maternal diabetes-induced perinatal programming, mediated in part by triggering the Nrf2-HO-1 defense system. PMID: 22733796 The increased expression of antioxidant catalase, specifically in hippocampal neurons, will provide protection from age-related cognitive decline. PMID: 21942371 The findings indicated that lower skin catalase activity in male mice may at least in part contribute to increased ultraviolet light B-induced generation of Gr-1+CD11b+ myeloid cells. PMID: 22030957 endogenous catalase plays an important role in protecting the kidney from diabetic stress through maintaining peroxisomal and mitochondrial fitness. PMID: 22315314 physiological venous catalase activity prevents exercise-induced up-regulation of endothelial nitric oxide synthase and extracellular superoxide dismutase PMID: 21129156 Data suggest that embryonic catalase plays an important protective role, and both physiological and phenytoin-enhanced oxidative stress can be embryopathic. PMID: 21478259 Chestnut inner shell extract treatment with ethanol decreased CYP2E1 expression and increased activities of catalase and superoxide dismutase. PMID: 21457746 Targeting catalase within mitochondria of tumor cells and tumor stromal cells suppresses ROS-driven tumor progression and metastasis. PMID: 21605372 The data indicate catalase levels is decreased in stem cell-like characteristics. PMID: 21347610 catalase may rescue paraquat-induced myocardial geometric and functional alteration possibly by alleviating JNK-mediated endoplasmic reticulum stress PMID: 20937379 There were no acute functional improvements with catalase overexpression before myocardial infarction, but sustained H(2)O(2), influenced by catalase expression, levels may be critical in directing remodeling and cardiac function at later time points. PMID: 20971939 The effect of increased mtDNA mutations on age-dependent cardiac structural changes and functional disturbance using Polgm/m mice and the contribution of mtreactive oxygen species in these mice by crossing Catalase to Polgm/m mice, was expored. PMID: 20456298 overexpression of catalase decreases oxidant injury in the proximal tubular cells reverses abnormalities due to enhanced generation of reactive oxygen species in the proximal tubule cells PMID: 20237455 peroxiredoxin-2, glutathione peroxidase-1, and catalase have roles in erythrocyte oxidative metabolism PMID: 19969073 we have demonstrated that forced catalase expression in the mitochondria enhances exercise performance. Our result provides a framework for further elucidating the underlying mechanism PMID: 19690612 A single base pair difference in the Cas1 open reading frame between the C57BL/6J and DBA/2J strains results in Ala-117 in the D2 strain and threonine at this position in the B6 strain. PMID: 11821648 Overexpression of catalase diminishes the pressor response to NE and Ang II by reducing H(2)O(2) production in the arterial wall. PMID: 12517675 The regulation of this gene's expression in mouse muscle cells is dependent on the CCAAT-binding factor NF-Y. PMID: 12659863 findings indicate that, in addition to stimulating catalase activity, c-Abl and Arg promote catalase degradation in the oxidative stress response PMID: 12950161 overexpression of catalase delays or prevents alterations in phospholipid metabolism during glucocorticoid-induced apoptosis PMID: 14572898 effect of IGFBP-2 overexpression on the activity of catalas in two malignant cell lines PMID: 14710363 the role of catalase in antioxidant defense is dependent on the type of tissue and the model of oxidant-mediated tissue injury PMID: 15178682

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.