Recombinant Mouse c-MET Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1342
Recombinant Mouse c-MET Protein (Fc Tag)

Recombinant Mouse c-MET Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1342
Catalog No.: BLPSN-1342

Product Overview

Tag Fc
Host Species Mouse
Accession NP_032617.2
Synonym AI838057, c-Met, HGF, HGFR, Par4
Background Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description A DNA sequence encoding the mouse MET (NP_032617.2) extracellular domain (Met 1-Asn 929) was fused with the Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Glu 25 & Ser 307
AA Sequence Met 1-Asn 929
Molecular Weight The recombinant mouse Met/Fc chimera is a disulfide-linked homodimer of the Met which is a heterodimer composed of the proteolytically cleaved alpha and beta subunits. Each alpha and beta together with the C-terminal Fc tag consists of 1146 a.a. and has a predicted molecular mass of 128 (alpha =32 + Fc tagged beta=96) kDa. The apparent molecular mass of the rm MET/Fc heterodimer thus is approximately 43 kDa and 115-120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
Purity >92% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its binding ability in a functional ELISA. Immobilized human HGF at 2 ug/ml (100 ul/well) can bind mouse HGFR with a linear ranger of 1.28-32ng/ml.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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