Recombinant Mouse c-MET Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1342
Recombinant Mouse c-MET Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1342
Catalog No.: BLPSN-1342
Product Overview
| Tag | Fc |
| Host Species | Mouse |
| Accession | NP_032617.2 |
| Synonym | AI838057, c-Met, HGF, HGFR, Par4 |
| Background | Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the mouse MET (NP_032617.2) extracellular domain (Met 1-Asn 929) was fused with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Glu 25 & Ser 307 |
| AA Sequence | Met 1-Asn 929 |
| Molecular Weight | The recombinant mouse Met/Fc chimera is a disulfide-linked homodimer of the Met which is a heterodimer composed of the proteolytically cleaved alpha and beta subunits. Each alpha and beta together with the C-terminal Fc tag consists of 1146 a.a. and has a predicted molecular mass of 128 (alpha =32 + Fc tagged beta=96) kDa. The apparent molecular mass of the rm MET/Fc heterodimer thus is approximately 43 kDa and 115-120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >92% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human HGF at 2 ug/ml (100 ul/well) can bind mouse HGFR with a linear ranger of 1.28-32ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
