Recombinant Mouse Brk Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0453
Recombinant Mouse Brk Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0453
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His&GST |
| Host Species | Mouse |
| Accession | Q64434 |
| Synonym | BRK, Sik, tks, Tksk |
| Background | Tyrosine kinase (PTKs) is a protein that carry out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demenstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis. |
| Description | A DNA sequence encoding the mouse PTK6 (Q64434) (Met1-Val451) was expressed with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met1-Val451 |
| Molecular Weight | The recombinant mouse PTK6/GST chimera consists of 688 a.a. and has a calculated molecular mass of 79.8 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | The specific activity was determined to be 5 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate. |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
