Recombinant Mouse Betacellulin / BTC Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0399
Recombinant Mouse Betacellulin / BTC Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0399
Catalog No.: BLPSN-0399
Product Overview
| Tag | His&Fc |
| Host Species | Mouse |
| Accession | NP_031594.1 |
| Synonym | Bcn |
| Background | Betacellulin(BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices; 3 residues)36% beta sheet (5 strands; 18 residues). BTC was originally identified as a growth-promoting factor in mouse pancreatic beta-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. |
| Description | A DNA sequence encoding the mouse BTC (NP_031594.1) extracellular domain (Met 1-Gln 118) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Asp 32 |
| AA Sequence | Met 1-Gln 118 |
| Molecular Weight | The recombinant mouse BTC/Fc is a disulfide-linked homodimer. The reduced monomer consists of 335 a.a. and has a predicted molecular mass of 38 kDa. As a result of glycosylation, the apparent molecular mass of rm BTC/Fc monomer migrates with an apparent molecular mass of 50-55 kDa in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.The ED50 for this effect is typically 2-10 ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
