Recombinant Mouse Annexin A2 (ANXA2) Protein (His&Myc)

Name: Recombinant Mouse Annexin A2 (ANXA2) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-00733P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins, Recombinant proteins fall special offers - full-length proteins

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Product Overview

Description	Recombinant Mouse Annexin A2 (ANXA2) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P07356
Target Symbol	ANXA2
Synonyms	(Annexin II)(Annexin-2)(Calpactin I heavy chain)(Calpactin-1 heavy chain)(Chromobindin-8)(Lipocortin II)(Placental anticoagulant protein IV)(PAP-IV)(Protein I)(p36)
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	STVHEILCKLSLEGDHSTPPSAYGSVKPYTNFDAERDALNIETAVKTKGVDEVTIVNILTNRSNVQRQDIAFAYQRRTKKELPSALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARELYDAGVKRKGTDVPKWISIMTERSVCHLQKVFERYKSYSPYDMLESIKKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
Expression Range	2-339aa
Protein Length	Full Length of Mature Protein
Mol. Weight	46.0 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.
Subcellular Location	Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane.
Protein Families	Annexin family
Database References	KEGG: mmu:12306 STRING: 10090.ENSMUSP00000034756 UniGene: PMID: 28536720 Our data indicate that hepatic overexpression of ANX1 and ANX2 inhibits APAP-induced expansion of liver injury PMID: 27451051 This study indicated that AnxA2 played complex roles in traversal of C. neoformans across host brain microvascular endothelial cells. PMID: 28130864 contributes to pulmonary microvascular integrity by enabling vascular endothelial cadherin-related phosphatase activity, thereby preventing vascular leak during alveolar hypoxia PMID: 28694388 our studies show that this G-motif represents a novel and essential determinant for axonal localization of the Anxa2 mRNA mediated by the SMN complex. PMID: 28258160 Despite comparable fungal burden, Anxa2(-/-) mice died more rapidly than wild-type mice when infected with Cryptococcus neoformans, and Anxa2(-/-) mice exhibited enhanced inflammatory responses PMID: 27371724 annexin A2 contributes to lung injury and fibrotic disease by mediating the fibrogenic actions of FXa. PMID: 28283478 Our findings reveal a previously undemonstrated function for AnxA2 in inflammatory response in polymicrobial sepsis models PMID: 27389701 Podxl-overexpression in neural stem/progenitor cells leads to an up-regulation of Annexin A2. PMID: 27470587 The tick protein sialostatin L2 binds to annexin A2 and inhibits NLRC4-mediated inflammasome activation. PMID: 27045038 Data show that annexin A2 (AnxA2) directly exerted negative regulation of inflammatory responses through Toll-like receptor 4 (TLR4)-initiated TRAM protein-TRIF protein pathway occurring on endosomes. PMID: 26527544 the role of Axna2 is instructive for understanding the development of RA, suppress the effect of Axna2 might provide a new potential measure for treatment of RA. PMID: 26963384 Taken together, these findings indicate for the first time that AnxA2 phosphorylation and actin remodeling evoked by oxidative stress depend on the sphingolipid pathway, via MMP2 and p38MAPK. PMID: 25574848 Data indicate that annexin A2 (AnxA2) regulates the secretion of semaphorin 3D (Sema3D) from pancreatic ductal adenocarcinoma (PDA) cells. PMID: 26243191 results suggest that legumain expression and activation and cleavage of annexin A2 are regulated by DJ-1 through p53 PMID: 26462467 AnxA2 regulates autophagy, thereby contributing to host immunity against bacteria through the Akt1-mTOR-ULK1/2 signaling pathway PMID: 26371245 These data suggest that AnxA2 and AnxA5 can influence bone formation via regulation of osteoprogenitor proliferation, differentiation, and responsiveness to cytokines in addition to their well-studied function in matrix vesicles PMID: 25222280 The tumor microenvironment interaction between ANXA2-CXCL12 is critical for metastatic phenotypes and may impact chemotherapeutic potential. PMID: 25139998 Our results support a model whereby AnxA2 limits the availability of TRPA1 channels to regulate nociceptive signaling in vertebrates. PMID: 25355205 Absence of ANXA2 leads to retention of COL6 in a late-Golgi, VAMP2-positive compartment. PMID: 24357721 data revealed a plausible new role of AnxA2 in the reduction of PCSK9 protein levels via a translational mechanism. PMID: 24808179 results suggest an involvement of AnxA2 in potentiating glutamate-induced cell death processes PMID: 24742684 Plasminogen stimulates autocrine cytokine production in human airway smooth muscle cells in a manner mediated by plasmin and annexin A2. PMID: 23721211 an annexin A2 niche in cell fate regulation PMID: 23148505 AnxA2 acts as an endogenous regulator of PCSK9-induced LDLR degradation PMID: 22848640 Tissue plasminogen activator activates the NF-kappaB pathway in macrophages through a signaling pathway involving annexin A2/CD11b-mediated integrin-linked kinase. PMID: 22677557 The results of the present study demonstrate the relevance of ANXII in vivo and suggest that methionine-induced hyperhomocysteinemia may reverse hyperfibrinolysis in APL. PMID: 22517898 results indicate a functional role of annexin A2 binding to endosomal membranes following organelle destabilization PMID: 22453828 ANXA2 redox regulatory function plays an important role protecting cells from oxidative stress, particularly during tumorigenesis. PMID: 22185818 Data demonstrate the presence of a binding site on the 3'-untranslated region of NR1 mRNA for AnxA2 and show the regulation of NR1 mRNA by AnxA2, GIIbeta and a third NR1 mRNA-binding protein, which is yet to be identified. PMID: 21995826 Transcriptional induction of the annexin A2 gene through the direct action of the hypoxia-inducible factor-1 complex is associated with proliferative phase of oxygen-induced retinopathy. PMID: 21788340 Lipid raft endocytosis and exosomal transport facilitate extracellular trafficking of annexin A2 PMID: 21737841 Data show that ANXA2 is part of a novel molecular pathway underlying PDA metastases and a new target for development of PDA therapeutics. PMID: 21572519 in endothelial cells, plasmin specifically induces activation of conventional PKC, which phosphorylates serine 11 and serine 25 of A2, triggering dissociation of the (A2.p11)(2) tetramer. PMID: 21115493 Annexin A2 expression is required for the biologic effects of progastrin in vivo and in vitro. PMID: 20826156 annexin-2 serves as an anchor for CXCL12 to help in the localization of HSCs to the niche PMID: 21108988 These data demonstrate that AnxA2 expression in osteoblasts is under the control of VEGF, which may have implications for both angiogenesis and bone mineralization under low oxygen conditions. PMID: 20817051 Annexin II mediates the binding of anti-dsDNA antibodies to mesangial cells, contributing to the pathogenesis of lupus nephritis. PMID: 20847146 Suggest that TM601, through its interaction with annexin A2, causes suppression and regression of ocular neovascularization and reduces vascular leakage. PMID: 20607799 The modulation of macrophage function by Annexin A2 tetramer is mediated through TLR4. PMID: 19965653 secretion caused by activation of insulin receptor and insulin-like growth factor receptor PMID: 12431980 annexins A1 and A2 bind to dysferlin PMID: 14506282 data establish annexin II as a regulator of cell surface plasmin generation and reveal that impaired endothelial cell fibrinolytic activity constitutes a barrier to effective neoangiogenesis PMID: 14702107 There was a marked increase in the ability of insulin to stimulate recruitment of cmyc-GLUT4 protein to the cell surface. PMID: 14726447 a novel functional role for the RNA-binding properties of annexin A2 in perinuclear localization of mRNA and the association with the cytoskeleton. PMID: 15654879 findings reveal that Anxa2 regulates HSC homing and binding to the bone marrow microenvironment and suggest that Anxa2 is crucial for determining the bone marrow niche of HSCs PMID: 17360942 From its characteristic distribution and molecular features, AX II may be important for myelin function in the PNS. PMID: 17549680 The effect of alcohol on fibrinolysis and plasmin is mediated in part by ANXA2. Alcohol directly influences hepatic pathways of fibrinolysis that may contribute to ALD. PMID: 18289715 Within the endothelial cell, p11 is required for Src kinase-mediated tyrosine phosphorylation of A2, which signals translocation of both proteins to the cell surface. PMID: 18434302 ANXA2 introduces the generation or expression of plasmin, S100A10, and fascin in tumor cells. ANXA2 enhances the development, invasion, and metastasis of tumor. PMID: 18608216

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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