Recombinant Mouse ANGPTL4 Protein (C-Fc)

Name: Recombinant Mouse ANGPTL4 Protein (C-Fc)
Brand: Beta LifeScience
SKU: BL-0236NP
Availability: InStock

BL-0236NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Angiopoietin Like Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys167-Ser410 is expressed with a human IgG1 Fc tag at the C-terminus.
Accession	Q9Z1P8
Synonym	Angiopoietin-related protein 4;425O18-1;Angiopoietin-like protein 4;Fasting-induced adipose factor;Hepatic fibrinogen/angiopoietin-related protein;HFARP;Secreted protein Bk89;Angptl4;Farp; Fiaf; Ng27
Gene Background	Angiopoietin-related protein 4（ANGPTL4）is a secreted protein and contains 1 fibrinogen C-terminal domain. The protein may act as a regulator of angiogenesis and modulate tumorigenesis. It inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 may exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity (By similarity). In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
Molecular Mass	54.6 KDa
Apmol Mass	67 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis (Probable).; Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
Subcellular Location	Secreted. Secreted, extracellular space, extracellular matrix.
Database References	KEGG: mmu:57875 STRING: 10090.ENSMUSP00000002360 UniGene: PMID: 28867683 Functional studies in Angptl4-deficient mice confirm improved insulin sensitivity and glucose homeostasis. PMID: 29899519 Data show that angiopoietin-like protein 4 (ANGPTL4)deficiency in mice knockout (ANGPTL4(-/-)) exacerbated colonic inflammation induced by dextran sulfate sodium (DSS) or stearic acid. PMID: 28287161 Angptl4 is induced early in fasting to divert uptake of fatty acids and triglycerides away from adipose tissues. PMID: 28752045 haematopoietic ANGPTL4 deficiency increases atherogenesis through regulating myeloid progenitor cell expansion and differentiation, foam cell formation and vascular inflammation. PMID: 27460411 Following ANGPTL4 downregulation, the proliferation and invasion abilities of gastric cancer (GC)cell lines were suppressed as determined by MTT and Transwell assays, and cell apoptosis level and sensitivity to cisplatin were increased as determined by flow cytometry and MTT assay. In conclusion, these findings suggest that ANGPTL4 may be a new potential therapeutic target for GC PMID: 29436683 The influence of H2O2 on ANGPTL4 provided new insight into the mechanism of atherosclerosis. PMID: 28849063 The reduction of plasma triglyceride levels in Angptl4(-/-) mice and increase following Angptl4 overexpression suggest that changes in plasma triglyceride metabolism do not regulate alpha-cells in the pancreas. Our findings corroborate recent data showing that increased plasma amino acids and their transport into alpha-cells link glucagon receptor blockage to alpha-cell hyperplasia PMID: 28143927 study demonstrates the key role of Angptl4 in glucocorticoid-augmented hepatic ceramide production that induces whole-body insulin resistance. PMID: 28743803 1) ANGPTL4 is not involved in the triglyceride-lowering effect ofbile acids ; 2) ANGPTL4 promotes bile acids absorption during taurocholic acid supplementation via a mechanism dependent on the gut microbiota. PMID: 28733267 physiological changes in adipose tissue ANGPTL4 expression during fasting and cold resulted in inverse changes in the amount of mature-glycosylated LPL in wild-type mice, but not Angptl4(-/-) mice. We conclude that ANGPTL4 promotes loss of intracellular LPL by stimulating LPL degradation after LPL processing in the endoplasmic reticulum (ER). PMID: 27034464 Angptl4-deficient mice show impaired insulin secretion and dysmorphic pancreatic islets. PMID: 28188788 Angptl4 induces obesity-associated metabolic disorders. The present study suggested that Angptl4 promotes liver steatosis and lipolysis, in addition to impairing liver function; while Angptl4 improves glucose tolerance and insulin resistance, in addition to causing the downregulation of various insulin signaling pathway-associated genes. PMID: 27573470 ANGPTL4 is part of a shuttling mechanism that directs fatty acids derived from circulating triglyceride-rich lipoproteins to brown adipose tissue during cold. PMID: 26476336 these results suggest that IL-1beta increases Angptl4 expression through a mechanism dependent on the JNK-MAPK signaling pathway in MC3T3-E1 cells. PMID: 26069075 glucagon receptor antagonist improves glycemia in diet-induced obese angptl4 knockout mice without increasing glucagon levels or alpha-cell proliferation, underscoring the importance of this protein. PMID: 26621734 This study showed that phloridzin improved plasma lipoprotein lipase activity via a decrease of ANGPTL4 mRNA expression and an increase of AMP-activated protein kinase phosphorylation. PMID: 24932810 Letter: Angiopoietin-like 4 is induced during sebocyte differentiation and regulates sebaceous lipogenesis in vitro but is dispensable for sebaceous gland function in vivo. PMID: 24815769 GR and FoxO1 are required for Angptl4 transcription activation, and that FoxO1 negatively mediates the suppressive effect of insulin PMID: 24565756 ANGPTL4 is a genetically and epigenetically inactivated secreted tumor suppressor that inhibits tumor angiogenesis. PMID: 23686315 inactivation of LPL by Angptl4 appears to occur after both proteins have traveled along the secretory pathway and arrived at the cell surface. PMID: 24220340 Angptl4 is necessary for rapid modulation of lipoprotein lipase activity in adipose tissue. PMID: 23176178 Depletion of adipose angiopoietin-like 4 abolishes intermittent hypoxia-induced dyslipidemia and atherosclerosis in mice, and is regulated by hypoxia-inducible factor-1. PMID: 23328524 Angptl4 suppresses foam cell formation to reduce atherosclerosis development. PMID: 23640487 Angptls would be useful in instances where there is a need to maintain HSCs ex vivo, such as during transduction for gene therapy applications PMID: 22639947 Locally expressed Angptl4 might play a role in local uterine/placental lipid metabolism. PMID: 22350948 The effect of Toll-like Receptor activation on the expression of macrophage ANGPTL4 was studied. PMID: 22538368 Angptl4 mRNA expression was increased through the elevated free FAs in diabetic mice. PMID: 22068616 Data showed that PPARbeta/delta regulates epidermal maturation via ANGPTL4-mediated signalling pathway. PMID: 21966511 ANGPTL4 tunes endothelial cell junction organization and pericyte coverage and controls vascular permeability and angiogenesis, both during development and in pathological conditions. PMID: 21832056 serum LDL, HDL and TG levels are decreased in LDLR-, Angptl- mice. PMID: 21549101 the cleavage of ANGPTL4 by these PCs modulates its inhibitory effect on LPL activity. PMID: 21398697 Angiopoietin-like 4 interacts with integrins beta1 and beta5 to modulate keratinocyte migration. PMID: 20952587 Angptl4 protects against severe proinflammatory effects of saturated fat by inhibiting fatty acid uptake into mesenteric lymph node macrophages. PMID: 21109191 Decreased fat storage by Lactobacillus paracasei is associated with increased levels of ANGPTL4. PMID: 20927337 Valsartan reduced fiaf gene expression in subcutaneous, but not visceral, fat in the ob/ob mouse. PMID: 20472602 Angptl4-null mice were resistant to diet-induced obesity, indicating obesity-promoting effects of Angptl4 under the condition of fat-enriched diet. PMID: 20798332 ANGPTL4 interacts with vitronectin and fibronectin in the wound bed, delaying their proteolytic degradation by metalloproteinases. PMID: 20729546 Stimulation of cardiac Angptl4 gene expression by dietary fatty acids and via PPARbeta/delta is part of a feedback mechanism aimed at protecting the heart against lipid overload and consequently fatty acid-induced oxidative stress. PMID: 20378851 These studies demonstrate that ANGPTL4 is a positive acute phase protein and the increase in ANGPTL4 could contribute to the hypertriglyceridemia that characteristically occurs during the acute phase response by inhibiting LPL activity. PMID: 20043872 potent hyperlipidemia-inducing factor in mice and inhibitor of lipoprotein lipase PMID: 12401877 FIAF may partially exert its function via a truncated form PMID: 15190076 angiopoietin-like protein 4 has an oligomerization state-dependent hyperlipidemic effect PMID: 15292369 Induction of Angptl4 in the heart inhibits lipoprotein-derived fatty acid delivery. PMID: 15659544 Differential regulation of Angptl3 & Angptl4 by sites of expression, nutritional status, & ligands of nuclear receptors may confer unique roles of each in lipoprotein metabolism. Angptl4 expression is activated by ligands of all PPAR. PMID: 15863837 Angptl4 is a potential angiogenic mediator in arthritis. PMID: 15870027 Angptl4-deficient mice had hypotriglyceridemia and increased postheparin plasma lipoprotein lipase, with greater effects in fasted state. Deficiecy in both Angptl proteins had additive effect on plasma triglycerides with survival not past 2 months of age. PMID: 16081640 via physical association with plasma lipoproteins, FIAF acts as a powerful signal from fat and other tissues to prevent fat storage and stimulate fat mobilization PMID: 16272564 First report of molecular cloning and characterization of ANGPT4in pigs, which will be helpful for a better understanding of the role of ANGPTLs in lipid metabolism. PMID: 16717449 Hypoxia/ischaemia rapidly increased fiaf mRNA in the injured cortex and hippocampus at 2 and 7 days after hypoxia/ischaemia. PMID: 16837853

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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Recombinant Mouse ANGPTL4 Protein (C-Fc)

Recombinant Mouse ANGPTL4 Protein (C-Fc)

Product Overview

Target Details

FAQs