Recombinant Mouse ACVR2A Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0071
Recombinant Mouse ACVR2A Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0071
Catalog No.: BLPSN-0071
Product Overview
| Tag | His&Fc |
| Host Species | Mouse |
| Accession | NP_031422.3 |
| Synonym | ActrIIa, Acvr2, TactrII |
| Background | ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways. |
| Description | A DNA sequence encoding the extracellular domain of mouse ACVR2A (NP_031422.3) (Met 1-Pro 134) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Ala 20 |
| AA Sequence | Met 1-Pro 134 |
| Molecular Weight | The secreted recombinant mouse ACVR2A/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 363 a.a. and has a calculated molecular mass of 41.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-65 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 20-60 ng/mL in the presence of 10 ng/mL recombinant Activin A. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
