Recombinant Mouse A33 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0027
Recombinant Mouse A33 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0027
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | NP_067623.1 |
| Synonym | 2010310L10Rik, 2210401D16Rik, BB116197 |
| Background | Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the mouse GPA33 (NP_067623.1) extracellular domain (Met 1-Ile 235) was expressed, with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Leu 22 |
| AA Sequence | Met 1-Ile 235 |
| Molecular Weight | The secreted recombinant mouse GPA33 consists of 225 a.a. and has a predicted molecular mass of 25.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmGPA33 is approximately 35-40 kDa due to glycosylation. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
