Recombinant Leishmania Infantum Thymine Dioxygenase Jbp1 (JBP1) Protein (His-V5)
Beta LifeScience
SKU/CAT #: BLC-01622P
Greater than 85% as determined by SDS-PAGE.
Recombinant Leishmania Infantum Thymine Dioxygenase Jbp1 (JBP1) Protein (His-V5)
Beta LifeScience
SKU/CAT #: BLC-01622P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Leishmania Infantum Thymine Dioxygenase Jbp1 (JBP1) Protein (His-V5) is produced by our E.coli expression system. This is a protein fragment. |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Uniprotkb | A4HU70 |
| Target Symbol | JBP1 |
| Synonyms | J-binding protein 1;Thymidine hydroxylase JBP1 |
| Species | Leishmania infantum |
| Expression System | E.coli |
| Tag | N-10His-V5 |
| Target Protein Sequence | SCIACLDGQFKGLALAFDDFGINVLMQPRDVMIFDSHHFHSNTEVELSFSGEDWKRLTCVFYYRAALGEPASYAEYRRRLEKSKQDTSFTPVVSNVRVKENGTNLNRPSPVYPIFLSPFWVPMVAHCLQHCASEAQCVHDAMTADGSRLAEVMFGEPLSTSDGIPLRGEEEKLKANSDSASRPLSRLGGFSETNLMVSTAVEKKKYLNSEFLSHFISAQLLDMWKQARGKWLELVGREWTHMLALNPERKDFLWRNQSEMNSAFFDLCEVGKQVMLGLLGKEAALPKEEQAFWTMYAVHLNAACAEELNMPHVAMSLRKLNVKLKDFNFGGTRYFKDMPPEEQKRRMERKQRIEEARRHGMSSGAHEKRANWLTNDSFDYQTEDCVFDYAQHKWVPPALHA |
| Expression Range | 200-600aa |
| Protein Length | Partial |
| Mol. Weight | 53.4 kDa |
| Research Area | Epigenetics And Nuclear Signaling |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
| Target Function | Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions. |
| Subcellular Location | Nucleus. |
| Protein Families | TET family, JBP1 subfamily |
| Database References | KEGG: lif:LINJ_09_1560 STRING: 435258.XP_001463611.1 |
![SDS-PAGE analysis of Human NDUFV1 (Nadh Dehydrogenase [Ubiquinone] Flavoprotein 1, Mitochondrial) - Recombinant Protein, CAT# BLT-08321P, showing >90% purity under 15% SDS-PAGE (Reduced)](http://www.betalifesci.com/cdn/shop/files/1ihmbkjr21vdmc6j_{width}x.jpg?v=1753363793)
