Recombinant Human Wnt3a V2 Protein

Name: Recombinant Human Wnt3a V2 Protein
Brand: Beta LifeScience
SKU: BL-2877NP
Availability: InStock

BL-2877NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality cytokines for advanced research, High-quality recombinant proteins

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Product Overview

Description	Recombinant Human Protein Wnt-3a is produced by our Mammalian expression system and the target gene encoding Ser19-Lys352 is expressed with a fusion design at the N-terminus.
Accession	P56704
Synonym	MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a
Gene Background	Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
Molecular Mass	105.7 kDa
Apmol Mass	110-155 kDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 10mM PB, 5% Sucrose, 0.01% Tween 80, pH7.4.
Endotoxin	Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
Subcellular Location	Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families	Wnt family
Database References	HGNC: 15983 OMIM: 606359 KEGG: hsa:89780 STRING: 9606.ENSP00000284523 UniGene: PMID: 30145863 High WNT3A expression is associated with colon cancer proliferation and metastasis. PMID: 29948330 Wnt3A regulates the expression of 1,136 genes, of which 662 are upregulated and 474 are downregulated in CCD-18Co cells. A set of genes encoding inhibitors of the Wnt/beta-catenin pathway stand out among those induced by Wnt3A, which suggests that there is a feedback inhibitory mechanism. PMID: 29044515 PCR and western blot results showed that inhibiting the secretion of Wnt3A blocked the Wnt signaling pathway and prevented Nrf2 signaling. Notably, the Wnt inhibitor may serve as a radiosensitizing drug PMID: 28627706 Study found that Wnt3a highly expressed in hepatocellular carcinoma (HCC) tissues, and confirmed that Wnt3a plays an essential role in HCC progression. Activation of the Wnt3a pathway is accompanied by higher expression of Notch3. Furthermore, Wnt3a may be critical for HCC cell cycle and metastasis by regulating cell cycle regulatory proteins and the MAPK pathway. PMID: 28902357 WNT3A supports early stages such as the ALP activity, but it does neither improve later stages of the osteogenic differentiation nor it inhibits the genuine adipogenic differentiation of adipose tissue derived mesenchymal stem cells. PMID: 28549605 SP5 negatively regulates WNT3a transcriptional programs in human pluripotent stem cells. PMID: 29044119 Suggest that GPC5 is able to suppress the lung adenocarcinoma metastasis by competitively binding to Wnt3a and inactivating the Wnt/beta-catenin signaling pathway. PMID: 27806326 WNT3A/beta-catenin signaling inhibition is involved in leflunomide-mediated cytotoxic effects on renal carcinoma cells PMID: 27391060 BRG1 may contribute to colon cancer progression through upregulating WNT3A expression. PMID: 27852072 Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems PMID: 27411105 study reveals that Foxi1/miR-491-5p/Wnt3a/beta-catenin signaling is critical in the progression of GC. Targeting the pathway described in this study may open up new prospects to restrict the progression of gastric cancer PMID: 28358374 CHIR suppressed the hypertrophic propensity of the MSC-derived cartilage after in vivo implantation to an extent approaching that of WNT3A protein. These results indicate that CHIR may be a promising alternative for WNT3A protein for certain applications of human bone marrow-derived MSCs. PMID: 27633010 PEGylated Wnt3A liposomes associated with skeletal stem cell populations in human bone marrow and promoted osteogenesis. PMID: 28351228 The AChE plays a role in osteoblastic differentiation and is regulated by both Wnt3a and Runx2. PMID: 28607150 In promoting the self-renewal symmetric division of hTERT(high) prostate cancer cells, WNT3a dramatically decreased the ratio of hTERT(high) prostate cancer cells undergoing asymmetric division. Increased WNT/beta-catenin signal activation was also detected in hTERT(high) prostate cancer cells. hTERT-mediated CSC properties were at least partially dependent on beta-catenin. PMID: 28209613 the results indicate neighboring structural elements within full-length Wnt3a affect saposin-like subdomain (SLD) conformational stability. Moreover, SLD function(s) in Wnt proteins appear to have evolved away from those commonly attributed to SAPLIP family members. PMID: 28754322 CPE through its N'-terminal sequence, forms aggregates with Wnt3a and possible endoplasmic reticulum (ER) stress leading to its loss of function. PMID: 27375026 Our findings suggest that Pyk2 plays an important role in the coordination of stabilization of beta-catenin in the crosstalk between Wnt/beta-catenin and Wnt/Ca(2+) signaling pathways upon Wnt3a stimulation in differentiating hNPCs. PMID: 28694190 Macrophage polarization seems to have a key role in the progression of pediatric non-alcoholic fatty liver disease; the modulation of macrophage polarization could drive hepatic progenitor cell response by Wnt3a production and beta-catenin phosphorylation. PMID: 27310371 this study shows that Wnt3a promotes differentiation of dendritic cells, but inhibits their maturation PMID: 27641635 Taken together, our results suggest that canonical Wnt signaling and its antagonist, sFRP1, regulate proliferation of human CSCs. Furthermore, excess sFRP1 in elderly patients causes CSC aging. PMID: 28435069 Thus, our findings provide mechanistic insight into the proposed cross-talk between the Wnt/beta-catenin and Hippo pathways in androgen-independent prostate cancer development. PMID: 28366633 TGF-beta1 signaling might have roles in myofibroblast differentiation in response to orthodontic force. We reveal in vitro that both Wnt3a and TGF-b1 promote myofibroblast differentiation from human periodontal ligament cells PMID: 28223136 Furthermore, pigment epithelium-derived factor (PEDF), a secreted glycoprotein known for its anti-tumor properties, blocked Wnt3a-directed induction of autophagy proteins. Autophagy inhibition was complemented by reciprocal regulation of the oxidative stress enzymes, superoxide dismutase 2 (SOD2) and catalase. PMID: 27557659 we performed a rescue experiment in the JB6 cell line and found that the inhibitory effect of Wnt5a on cell proliferation could be rescued by the addition of Wnt3a. Our data reveal that Wnt5a suppresses the activation of b-catenin signaling during hair follicle regeneration. PMID: 27499692 The level of Wnt3a expression in hepatocellular carcinoma(HCC) patients was obviously higher than that in any group of cases with benign liver diseases. The diagnostic specificity or the area under the receiver operating characteristic curve was 94.34 % or 0.994 in Wnt3a and 69.81 % or 0.710 in AFP for HCC, respectively. PMID: 26577850 Oncogenic Wnt3a expression associated with HBV infection and cirrhotic liver might be an independent prognostic factor for hepatocellular carcinoma. PMID: 27076768 AurkA suppresses the expression of miR-128, inhibitor of wnt3a mRNA stabilization. PMID: 27341528 The pool of ADP-ribosylated Axin, which is degraded under basal conditions, increases immediately following Wnt stimulation in both Drosophila and human cells. PMID: 27138857 The results reveal a novel inhibitory role of Wnt3a on canonical Wnt/beta-catenin signaling and cancer cell proliferation when there is an insufficient blood supply during tumor development. PMID: 26643293 Upregulated Wnt3a in colon cancer cells promoted the capacity to form tube-like structures in the three-dimensional (3-D) culture together with increased expression of endothelial phenotype-associated proteins such as VEGFR2 and VE-cadherin. PMID: 26266404 72 Wnt target genes higher expressed in triple-negative breast cancer PMID: 25848952 a Wnt-amplified environment was associated with superior pulp healing. PMID: 25556760 Wnt3a can modulate intracellular localisation and secretion of sFRP4. PMID: 25805505 These observations demonstrated the first time that Wnt3a can directly activate MyoD expression through targeting cis-elements in the DE and the L fragment. PMID: 25651906 Wnt-mediated protein stabilization ensures proper mitotic microtubule assembly and chromosome segregation. PMID: 25656539 Wnt3a expression was associated with epithelial mesenchymal transition and promoted colon cancer progression. PMID: 25499541 Wnt3a and Notch3 may promote the metastasis of non-small cell lung cancer and Notch3 upregulation is required for the Wnt3a mediated increased metastatic abilities of non-small cell lung cancer. PMID: 25572698 SNP rs752107 in WNT3A was strongly associated with decreased total hip BMD showing the highest significance under the recessive model PMID: 24584697 miR-195 is key in regulating cell proliferation, cell cycle and apoptosis through targeting Wnt3a. PMID: 25174704 WNT3A alone was a poor inducer of mesoderm from hESCs in defined medium. WNT3A and BMP4 synergized to accelerate mesoderm formation. Added later, WNT3A blocked hematopoiesis and generated mesenchymal colonies. PMID: 24052942 High WNT3A expression is associated with glioblastoma. PMID: 25301448 WNT3A gene expression is associated with isolated Hirschsprung disease polymorphism and disease status. PMID: 24817932 High WNT3A expression is associated with bone destruction in lung and breast cancer. PMID: 25359619 High Wnt3A expression is associated with metastasis in triple negative breast cancer. PMID: 24209998 Application of recombinant Wnt3a showed an anti-proliferative effect on keratinocytes in a dose-dependent manner. PMID: 24686518 these results show that TAZ mediates Wnt3a-stimulated osteogenic differentiation through PP1A, suggesting that the Wnt signal regulates the Hippo pathway. PMID: 24510127 The LRP6 tyrosine mutant increased in signaling activation in response to Wnt3a/beta-catenin stimulation. PMID: 25143377 p66(Shc) plays a vital part in canonical Wnt signaling in the endothelium and mediates Wnt3a-stimulated endothelial oxidative stress and dysfunction. PMID: 25147340

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Human Wnt3a V2 Protein

Recombinant Human Wnt3a V2 Protein

Product Overview

Target Details

FAQs