Recombinant Human VSIG4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4840
Recombinant Human VSIG4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4840
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Human |
Accession | O75144 |
Synonym | CRIg, Z39IG |
Background | VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type Ig domain and a truncated C-type Ig domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, an V-type Ig domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type Ig domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type Ig domains of rat and human VSIG4, respectively. |
Description | A DNA sequence encoding the human VSIG4 (O75144-1) (Met1-Pro283) was expressed with the Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Arg 20 |
AA Sequence | Met1-Pro283 |
Molecular Weight | The recombinant human VSIG4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 505 a.a. and has a predicted molecular mass of 56.2 kDa. The apparent molecular mass of the protein is approximately 57 kDa in SDS-PAGE under reducing conditions. |
Purity | (97.2+1.4)% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. |
Subcellular Location | Cell membrane; Single-pass type I membrane protein. |
Protein Families | Immunoglobulin superfamily, BTN/MOG family |
Database References | |
Tissue Specificity | Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leuko |