Recombinant Human Von Willebrand Factor Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4835
Recombinant Human Von Willebrand Factor Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4835
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_000543.2 |
Synonym | F8VWF, Von Willebrand Factor, VWD |
Background | Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on. |
Description | A DNA sequence encoding the pro form of human von Willebrand factor (NP_000543.2) (Met 1-Lys 2813) was expressed with a C-terminal His tag. |
Source | CHO Stable Cells |
Predicted N Terminal | Ala 23 |
AA Sequence | Met 1-Lys 2813 |
Molecular Weight | The secreted recombinant human vWF consists of 2802 a.a. after removal of the signal peptide and has a predicted molecular mass of 308 kDa. Purified rhvWf exists as both the pro form with the pro peptide (307 kDa) and the mature form (226 kDa), which migrates as doublets with apparent molecular mass of 260 and 350 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation. |
Purity | >75% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |