Recombinant Human V-Type Proton Atpase Subunit G 1 (ATP6V1G1) Protein (GST)

Name: Recombinant Human V-Type Proton Atpase Subunit G 1 (ATP6V1G1) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-08540P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human V-Type Proton Atpase Subunit G 1 (ATP6V1G1) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O75348
Target Symbol	ATP6V1G1
Synonyms	ATP 6G; ATP 6J; ATP6G; ATP6G1; ATP6GL; Atp6v1g1; ATPase H+ transporting lysosomal (vacuolar proton pump) member J; ATPase H+ transporting lysosomal 13kD V1 subunit G isoform 1; ATPase H+ transporting lysosomal 13kDa V1 subunit G1; ATPase H+ transporting lysosomal member J; ATPase H+ transporting lysosomal subunit G isoform 1; DKFZp547P234; V ATPase 13 kDa subunit 1; V ATPase G subunit 1; V-ATPase 13 kDa subunit 1; V-ATPase subunit G 1; V-type proton ATPase subunit G 1; Vacuolar ATP synthase subunit G 1; Vacuolar ATP synthase subunit G1; Vacuolar ATP synthase subunit M16; Vacuolar H+ ATPase G1; Vacuolar H+ ATPase subunit G 1; Vacuolar proton pump G subunit 1; Vacuolar proton pump subunit G 1; Vacuolar proton pump subunit M16; VATG1_HUMAN; Vma 10; Vma10
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	ASQSQGIQQLLQAEKRAAEKVSEARKRKNRRLKQAKEEAQAEIEQYRLQREKEFKAKEAAALGSRGSCSTEVEKETQEKMTILQTYFRQNRDEVLDNLLAFVCDIRPEIHENYRING
Expression Range	2-118aa
Protein Length	Full Length of Mature Protein
Mol. Weight	40.6kDa
Research Area	Transport
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.
Subcellular Location	Apical cell membrane.
Protein Families	V-ATPase G subunit family
Database References	HGNC: 864 OMIM: 607296 KEGG: hsa:9550 STRING: 9606.ENSP00000363162 UniGene: PMID: 26180254 genome-wide association study in population in China: Data suggest 4 SNPs upstream of ATP6V1G1 (rs10817638, rs7851259, rs10982287, rs4979427) are associated (in bivariate manner) with age at menarche and bone density. [includes META-ANALYSIS] PMID: 26312577 RILP regulates the activity of the V-ATPase through its interaction with V1G1. PMID: 24762812

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.