Recombinant Human Uteroglobin Protein (His Tag)

Name: Recombinant Human Uteroglobin Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4755
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	P11684
Synonym	CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1
Background	Uteroglobin (UG), also known as Secretoglobin 1A member 1 (SCGB1A1), Blastokinin, Clara cell secretor protein (CCSP) or Clara cell-specific 1-kDa protein (CC1), is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is mainly expressed in lung, with anti-inflammatory/immunomodulatory properties. Previous in vitro studies demonstrated that CCAAT/enhancer-binding proteins (C/EBPs) are the major transcription factors for the regulation of SCGB1A1 gene expression, whereas FOXA1 had a minimum effect on the transcription. Uteroglobin is a multifunctional protein with antiinflammatory/immunomodulatory properties. Uteroglobin inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, Uteroglobin manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities. The tissue-specific expression of the Uteroglobin gene is regulated by several steroid hormones, although a nonsteroid hormone, prolactin, further augments its expression in the uterus. Based on its anti-inflammatory and antiallergic properties, Uteroglobin is a potential drug target. The mechanism of Uteroglobin action is likely to be even more complex as it also functions via a putative receptor-mediated pathway.
Description	A DNA sequence encoding the human SCGB1A1 (P11684) (Met 1-Asn 91) was expressed, with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Glu 22
AA Sequence	Met 1-Asn 91
Molecular Weight	The recombinant human SCGB1A1 consists of 81 a.a. and migrates as a 9.2 kDa band as predicted in SDS-PAGE under reducing conditions.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cell line. When 5 x 10E4cells/well are added to human SCGB1A1 coated plates (2 ug/ml and 100 ul/well), approximately >30% will adhere after one hour at 37 °C.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
Subcellular Location	Secreted.
Protein Families	Secretoglobin family
Database References	HGNC: 12523 OMIM: 192020 KEGG: hsa:7356 STRING: 9606.ENSP00000278282 UniGene: Hs.523732
Tissue Specificity	Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).

Gene Functions References

There was no evidence that serum CC16 levels played a role in recurrent wheezing and a positive asthma predictive index in pre-school children PMID: 29685782
Data suggest that the increase in plasma club cell protein (CC16) CC16 following inhaled allergen as a biomarker of epithelial dysfunction. PMID: 28862880
We propose that increased CC-16 levels is a marker of lung inflammatory response that associated with ventilatory insufficiency are related to impending respiratory failure, not fully predicted by conventional respiratory tests PMID: 28967121
Studied serum CC16 levels in The serum CC16 levels in Acute respiratory distress syndrome (ARDS) and non-ARDS patients; found CC16 to be were significantly higher in ARDS patients than that in non-ARDS patients. PMID: 28548310
Studied serum CC16 levels in relation to atopy and previously manifested low respiratory tract diseases. Found low serum CC16 is associated with previously expressed pneumonia and chronic wheezing in atopic children. PMID: 28146340
Our data suggest that rCC16 suppresses LPS-mediated inflammatory mediator TNF-alpha, IL-6, and IL-8 production by inactivating NF-kappaB and p38 MAPK but not AP-1 in RAW264.7 cells. PMID: 28338974
There were associations between urinary CC16 and arsenic concentration in soil, water, house dust, and dust loading. In multiple analyses, only the concentration of arsenic in soil was associated with urinary CC16 levels after accounting for other factors. PMID: 27223295
findings demonstrate that CC16 is upregulated in IPF patients suggesting that may participate in its pathogenesis. PMID: 27977812
The G38A CCSP gene polymorphism may alter either the production of the protein and/or its activity in chronic obstructive pulmonary disease. PMID: 27496897
CC16 levels differed among idiopathic pulmonary fibrosis, pulmonary sarcoidosis and chronic pulmonary obstructive disease. PMID: 27758987
Lung-specific (CC-16) and novel (RelB) biomarkers are associated with systemic cardiovascular changes over time. CC-16 can predict subsequent exacerbations in subjects with severe COPD and may be an important biomarker of pulmonary and systemic stress in COPD. PMID: 26914709
The serum concentration of CC16 was significantly higher in patients with lung fibrosis. PMID: 25244495
lower levels of urine CC16 and lung function in patients with asthma than in those patients without asthma. CC16 in urine may be a useful tool or biomarker for investigating lung epithelium integrity among children with asthma or lung injury. PMID: 26108072
Smokers and COPD patients had reduced airway CC16 immunostaining that decreased with increasing COPD severity. PMID: 25700379
the uteroglobin G38A gene polymorphism was not associated with IgAN risk PMID: 25068828
Sputum and bronchoalveolar lavage fluid CC16 were significantly higher in patients with severe asthma compared to mild-moderate asthma and healthy controls. PMID: 25728058
In chromium-exposed workers, blood levels of CC16, and CC16/SP-D were lower than in controls. Positive relationships were shown between CC16 or CC16/SP-D and indicators of lung function. PMID: 25851191
KL-6 levels were higher and CC16 levels were lower in infants with poor neurodevelopmental outcome compared with those infants who had favourable neurodevelopmental outcome. PMID: 25631862
CC16 may play an important protective role in cigarette smoke-related diseases. PMID: 25635997
A meta-analysis indicated that the CC16 gene A38G polymorphism is not associated with the risk of asthma. PMID: 25743006
Increased plasma clara cell secretory protein levels are associated with primary graft dysfunction. PMID: 24400993
Uteroglobin is a possible ligand of the lipoxin receptor and it may have a role in inhibiting serum amyloid A-driven inflammation PMID: 24782597
Athletes with decreased serum CC16 from regular high-load exercise are more susceptible to respiratory infections. PMID: 24735334
A single therapeutic dose of terbutaline offers significant protection against hyperpnoea-induced bronchoconstriction and reduces urinary CC16 protein levels. PMID: 24030662
Lung permeability biomakers [surfactant protein D (SP-D) and Clara cell secretory protein (CC16) in plasma] and forced expiratory volumes and flow were measured in swimmers in indoor swimming pool waters treated with different disinfection methods. PMID: 23874631
Serum CC-16 is associated with disease progression in chronic obstructive pulmonary disease (COPD). However, the absence of CC-16 does not appear to modify the risk of cigarette-related COPD in mice. PMID: 24245748
Acute exposure to smoke induces injury at the alveolar level, which results in a transient increase of CC16 in serum of exposed subjects. PMID: 23258467
These results suggest that the CC16 A38G polymorphism may play a role in asymptomatic airway hyper-responsiveness and contribute to the development of late-onset asthma. PMID: 24125144
The SCGB1A1 +38A/G polymorphism is a risk factor for asthma. [Meta-analysis] PMID: 23820082
urinary CC16 may be a useful biomarker of increased lung epithelial permeability among female non-smokers; further work will be required to evaluate its applicability to males PMID: 22805990
Clara cell secretory protein expression changes is a relevant marker in the development of bronchiolitis obliterans patients with lung transplantation. PMID: 22883104
Overview of CC16 in pathophysiology of and as a biomarker in chronic obstructive pulmonary disease. PMID: 23030587
A Genome wide association study for COPD biomarkers on subjects with COPD found 2 discrete loci affecting CC16, one near the CC16 coding gene (SCGB1A1) on chromosome 11 and one approximately 25Mb away from SCGB1A1, identified in expressed sputum. PMID: 23144326
Donor CCSP A38G polymorphism is associated with decreased CCSP levels early after lung transplantation and poor long-term outcomes. PMID: 22902791
Exercise caused an increase in urinary excretion of CC16 in all subjects (P < 0.001), but this rise in CC16 was blunted following inhalation of warm humid air. PMID: 21799131
demonstration that cc-10 is differentially expressed in infants with iRDS may point the way towards one possible mechanism that potentially involves modifications of the protein structure with its anti-inflammatory and surfactant protective function PMID: 22613976
CC10 G+38A variant may contribute to the severity of asthma and lower level of steroid responsiveness. PMID: 22788242
These results indicate that CC10 gene transfer may inhibit airway inflammation through suppressing the activation of NF-kappaB. PMID: 22558282
Serum Clara cell secretory protein levels were characterized by an early postnatal surge. This apparent gestation-influenced surge may represent an initiation of a protective cascade against postnatal lung injury during extrauterine adaptation. PMID: 21952535
Single-nucleotide polymorphism in the CC10 gene (A38G) does not seem to be involved in the severity of respiratory syncytial virus infection or wheezing. PMID: 21767304
Urinary levels of CC16 are increased after eucapnic voluntary hyperpnea. PMID: 21131866
Elevated circulating CC16 levels identified severe thoracic injury combined with a strong correlation with the extent/volume of affected lung tissue. PMID: 21045740
CC16 38A/38A genotype plays a role in the development of early asthma in children with AR. PMID: 21255142
Elevated plasma clara cell secretory protein concentration is associated with high-grade primary graft dysfunction. PMID: 21299834
In the context of allergic airway responses, CC10 can inhibit OPN expression and suppress the Th2-promoting function of OPN, resulting in CC10's inhibitory biological effects. PMID: 20553297
Data suggest that a supernatant of non-small-cell lung cancer causes an imbalance in the immune response of PBMCs and DCs, which is reversed by CC-10. PMID: 20664959
Letter: serum CCSP cannot be used as a biomarker predictive for bronchiolitis obliterans after lung transplantation. PMID: 20683434
Reduced anti-inflammatory CC10 concentrations in airways of extremely premature infants with a fetal inflammatory response, not umbilical cord serum CC10, might make their lungs susceptible for further postnatal injuries. PMID: 19887851
Association of CC16 with daily outdoor particulate matter from combustion sources increases epithelial barrier permeability in lungs. PMID: 19852548
The effect of G38A may be apparent under stimulation as sex steroids or infections, and homozygotes of the G38A mutation cannot produce sufficient UG in response to stimulation and may be predisposed to IgA nephropathy, especially in childhood. PMID: 11774099

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