Recombinant Human USP14 Protein (N-6His)

Name: Recombinant Human USP14 Protein (N-6His)
Brand: Beta LifeScience
SKU: BL-1273NP
Availability: InStock

BL-1273NP: Greater than 85% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase 14 is produced by our E.coli expression system and the target gene encoding Asp91-Gln494 is expressed with a 6His tag at the N-terminus.
Accession	P54578
Synonym	Ubiquitin Carboxyl-Terminal Hydrolase 14; Deubiquitinating Enzyme 14; Ubiquitin Thioesterase 14; Ubiquitin-Specific-Processing Protease 14; USP14; TGT
Gene Background	Ubiquitin Carboxyl-Terminal Hydrolase 14 (USP14) belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 located in the cytoplasm is a proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. In addition, USP14 is indispensable for synaptic development and function at neuromuscular junctions, required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis.
Molecular Mass	48.45 KDa
Apmol Mass	52 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 20% Glycerol, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 85% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.
Subcellular Location	Cytoplasm. Cell membrane; Peripheral membrane protein.
Protein Families	Peptidase C19 family, USP14/UBP6 subfamily
Database References	HGNC: 12612 OMIM: 607274 KEGG: hsa:9097 STRING: 9606.ENSP00000261601 UniGene: PMID: 29581427 Usp14 and Ube3c cycle together on and off proteasomes, and the presence of ubiquitinated substrates promotes their association. This mechanism enables proteasome activity to adapt to the supply of substrates. PMID: 28396413 Study detected elevated USP14 expression at both mRNA and protein levels, and was significantly associated with distant metastasis. PMID: 28509417 These findings suggested that USP14 induces NF-kappaB activity and ERK1/2 phosphorylation triggered by microbial infection. PMID: 28364380 Ubiquitin-specific protease 14 regulates cell proliferation and apoptosis in oral squamous cell carcinoma PMID: 27592452 our report demonstrates significant value in targeting USP14/UCHL5 with VLX1570 in drug-resistant Waldenstrom macroglobulinemia (WM) and carries a high potential for clinical translation PMID: 27813535 USP14 promotes prostate cancer progression. PMID: 28151478 without a ubiquitinated substrate present, Usp14 suppresses multiple proteasomal activities, especially basal ATP consumption and degradation of non-ubiquitinated proteins. These allosteric effects thus reduce ATP hydrolysis by inactive proteasomes and nonspecific proteolysis and enhance proteasomal specificity for ubiquitinated proteins. PMID: 28416611 USP14 regulates autophagy by negatively controlling K63 ubiquitination of Beclin 1 PMID: 27542828 USP14 plays an important role in the progression and metastasis of esophageal squamous cell carcinoma. PMID: 27629392 USP14 participates in CAM-DR of MM through acting as a bridge between Bcl-xl apoptotic pathway and Wnt-signaling pathways and may be represented as a good candidate for pursuing clinical trials in MM. PMID: 26710889 Results show that USP14 mRNA is overexpressed in hepatocellular carcinoma (HCC) tissues and inversely correlates with miR-4782-3p level which binds to USP14 3'UTR to regulate its expression. PMID: 26782643 Results found USP14 amplification and overexpression in many different cancers. Its overexpression in low-expression cell lines promoted cell proliferation and migration, whereas its downregulation suppressed tumor cell proliferation, increased apoptosis rate, and decreased cell migration and invasion suggesting an oncogenic role in various types of cancer. PMID: 26938858 Increased USP14 expression in patients with breast cancer was associated with a poorer prognosis. PMID: 26712154 Akt-mediated phosphorylation of USP14 at Ser432, which normally blocks its catalytic site in the inactive conformation, activates its deubiquitinating activity in vitro and in cells. PMID: 26523394 our findings suggested that USP14 is involved in the progression of hepatocellular carcinoma(HCC) and may be a useful therapeutic target in HCC PMID: 26397990 Cell surface ubiquitination precedes endocytosis, after which USP14 acts as an ubiquitin-binding protein that targets the ubiquitinated GABA B receptor to lysosomal degradation and promotes its deubiquitination. PMID: 26817839 USP14 shows a marked preference for ubiquitin-cyclin B conjugates that carry more than one ubiquitin modification or chain PMID: 27074503 our findings suggest that USP14 is involved in the progression of epithelial ovarian cancer PMID: 25429837 Downregulation of USP14 expression arrested the cell cycle, which may be related to beta-catenin degradation. PMID: 23702845 b-AP15 is an inhibitor of deubiquitylating enzyme USP14 and UCHL5 induces apoptosis in multiple myeloma and overcomes bortezomib resistance. PMID: 24319254 overexpression of HA-USP14 increased the LPS-, TNFalpha-, or Escherichia coli-induced IL-8 release in human lung epithelial cells. PMID: 23615914 the decrease in proteolysis of proteasomal substrates during aging is independent of the increased USP14 activity and that the reciprocal regulation of USP14 and proteasomal catalytic activity may be necessary to maintain cellular ubiquitin homeostasis. PMID: 23291607 High Ubiquitin-specific protease 14 expression associated with intrahepatic cholangiocarcinoma cell differentiation. PMID: 21627382 the catalytic cleft leading to the active site of USP14 is blocked by two surface loops. Binding by ubiquitin induces a significant conformational change thereby allowing access of the ubiquitin C-terminus to the active site. PMID: 16211010 Results show the overall survival rate was worse in patients with a high USP14/TGT60 kD expression level and suggest that USP14/TGT60 kD also controls the fate of proteins that regulate tumor invasion and metastasis. PMID: 16465409 Neuronal expression of full-length USP14 is able to restore the levels of monomeric ubiquitin in the brains of transgenic ataxia mice. PMID: 17079671 USP14 were over-expressed in ovarian serous cystadenocarcinoma tissues, suggesting that the activity of ubiquitin-proteasome system is obviously enhanced in ovarian cancer. PMID: 17553343 These findings suggest that USP14 is a novel player in the unfolded protein response by serving as a physiological inhibitor of ER-associated degradation under the non-stressed condition. PMID: 19135427 Data from transgenic mice define a critical role for Usp14 at mammalian synapses and suggest a requirement for local ubiquitin recycling by the proteasome to control the development and function of neuromuscular junctions. PMID: 19726649

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Human USP14 Protein (N-6His)

Recombinant Human USP14 Protein (N-6His)

Product Overview

Target Details

FAQs