Recombinant Human UCHL1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4721
Recombinant Human UCHL1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4721
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_004172.2 |
| Synonym | HEL-117, NDGOA, PARK5, PGP9.5, PGP95, Uch-L1 |
| Background | Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons. |
| Description | A DNA sequence encoding the native human UCHL1 (NP_004172.2) (Gln 2-Ala 223) was expressed, with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Gln 2-Ala 223 |
| Molecular Weight | The recombinant human UCHL1 consisting of 229 a.a. and migrates as an 25.6 kDa band in SDS-PAGE under reducing conditions as predicted. |
| Purity | >92% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/ug. |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
