Recombinant Human UBE2D1 Protein (GST Tag)

Beta LifeScience SKU/CAT #: BL-0256NP
Recombinant Human UBE2D1 Protein (GST Tag)

Recombinant Human UBE2D1 Protein (GST Tag)

Beta LifeScience SKU/CAT #: BL-0256NP
Catalog No.: BL-0256NP

Product Overview

Tag GST
Host Species Human
Accession P51668
Synonym UBE2D1 Ubiquitin-conjugating enzyme E2 D1;Stimulator of Fe transport;SFT;UBC4/5 homolog;UbcH5;Ubiquitin carrier protein D1;Ubiquitin-conjugating enzyme E2(17)KB 1;Ubiquitin-conjugating enzyme E2-17 kDa 1;Ubiquitin-protein ligase D1;SFT; UBC5A; UBCH5; UBCH5A
Background Ubiquitin-conjugating enzyme E2 D1(UBE2D1)belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Description Recombinant Human Ubiquitin-conjugating enzyme E2 D1 is expressed by our E.coli expression system and the target gene encoding Met1-Met147 is expressed with a GST tag at the N-terminus.
Source E.coli
AA Sequence Met1-Met147
Purity >90% as determined by reducing SDS-PAGE.
Endotoxin <0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Recombinant Human UBE2D1 Protein was lyophilized from a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerin, pH 7.5.
Reconstitution Dissolve the lyophilized protein in distilled water.
Usage For Research Use Only
Storage Human UBE2D1 protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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