Recombinant Human Tumor Necrosis Factor-Inducible Gene 6 Protein (TNFAIP6) Protein (His)

Beta LifeScience SKU/CAT #: BLC-03125P
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SDS-PAGE.

Recombinant Human Tumor Necrosis Factor-Inducible Gene 6 Protein (TNFAIP6) Protein (His)

Beta LifeScience SKU/CAT #: BLC-03125P
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Product Overview

Description Recombinant Human Tumor Necrosis Factor-Inducible Gene 6 Protein (TNFAIP6) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity Greater than 90% as determined by SDS-PAGE.
Uniprotkb P98066
Target Symbol TNFAIP6
Synonyms Hyaluronate binding protein; Hyaluronate-binding protein; TNF alpha induced protein 6; TNF alpha-induced protein 6; TNF stimulated gene 6 protein; TNF-stimulated gene 6 protein; TNFAIP 6; Tnfaip6; TSG 6; TSG-6; TSG6_HUMAN; Tumor necrosis factor alpha induced protein 6; Tumor necrosis factor alpha inducible protein 6; Tumor necrosis factor alpha-induced protein 6; Tumor necrosis factor inducible gene 6 protein; Tumor necrosis factor stimulated gene 6 protein; Tumor necrosis factor-inducible gene 6 protein
Species Homo sapiens (Human)
Expression System E.coli
Tag N-6His
Target Protein Sequence WGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL
Expression Range 18-277aa
Protein Length Full Length of Mature Protein
Mol. Weight 33.1kDa
Research Area Cell Adhesion
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.
Database References

HGNC: 11898

OMIM: 600410

KEGG: hsa:7130

STRING: 9606.ENSP00000243347

UniGene: PMID: 29401724

  • TSG-6 from MSCs attenuated severe burn-induced excessive inflammation via inhibiting activation of P38 and JNK signaling. PMID: 27444207
  • TSG-6 secreted by human mesenchymal stem cells suppresses inflammatory reactions through p38 and ERK in the mitogen-activated protein kinase pathway PMID: 28247086
  • This review will focus on the potential of mesenchymal stem/stromal cells for treatment of fibrotic diseases, with emphasis on the role of TSG-6 as a mediator of anti-inflammatory effects PMID: 26807758
  • abnormally elevated in both the plasma and aortic wall of patients with abdominal aortic aneurysms PMID: 29180197
  • Single nucleotide polymorphism in TNFAIP6 gene is associated with Systemic lupus erythematosus. PMID: 28246883
  • TNFAIP6 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  • Data show that TNF-stimulated gene-6 (TSG-6) interacts with chemokines through their glycosaminoglycans (GAGs)-binding sites and inhibits their binding to GAGs and endothelial cell surfaces. PMID: 27044744
  • Data postulate that the molecular cross-linking enhanced by the multiple binding modes of the Link module might be critical for remodeling the ECM during inflammation/ovulation and might contribute to other functions of TSG-6. PMID: 26685054
  • the well characterized hyaluronan-binding site in the TSG-6 Link module is not used for recognition during transfer of HCs onto HA. PMID: 26468290
  • TSG-6 exhibited anti-inflammatory effects during the wound healing process and cicatrization and significantly diminished hypertrophic scar formation in a rabbit ear model PMID: 25661977
  • results suggest that systemic administration of hASC or TSG-6 may be novel approaches to reverse CS-induced myelosuppression. PMID: 25329668
  • TSG-6 activity in tissues, was examined. PMID: 25325979
  • TSG-6 expression by hMSCs was induced 12 h after oropharyngeal delivery to LPS-exposed lungs. Knockdown of TSG-6 expression in hMSCs by RNA interference abrogated most of their anti-inflammatory effects. PMID: 21569482
  • Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan. PMID: 25561734
  • data suggest that TSG-6 expression might be essential in endometrial matrix organization and feto-maternal communication during the implantation process. PMID: 24213015
  • the HA-binding site defined here may not play a role in TSG-6-mediated transfer of heavy chains from inter-alpha-inhibitor onto HA, a process known to be essential for ovulation. PMID: 24403066
  • TSG-6 suppresses CXCL8-mediated chemotaxis of neutrophils; this lower potency effect might be important at sites where there is high local expression of TSG-6. PMID: 24501198
  • This study provides insight into what we believe to be a previously undescribed multifaceted role of mesenchymal stem cell-released TSG-6 in wound healing. PMID: 23921952
  • Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI) and that this effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells. PMID: 24005673
  • The protein product of tumor necrosis factor-alpha stimulated gene-6 strongly correlated with ossification markers and hyaluronidase in calcified human aortic valves. PMID: 23017666
  • This review discusses TSG-6 as a potential molecular marker of oocyte maturation. PMID: 22922154
  • TSG-6 increases the accumulation of HA in the cell-associated matrix, partially by preventing its dissolution from the cell-associated matrix into the conditioned medium, but primarily by inducing HA synthesis PMID: 23129777
  • analysis of irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6 PMID: 23166324
  • TSG-6 reverts the inhibitory effects exerted by PTX3 on FGF2-mediated angiogenesis through competition of FGF2/PTX3 interaction. PMID: 22267482
  • TSG-6 was central to EMT through effects on HA macromolecular structure and through CD44-dependent triggering of cell responses. PMID: 21864707
  • TSG-6 is a potent HA cross-linking agent PMID: 21596748
  • TSG-6 protein, a negative regulator of inflammatory arthritis, forms a ternary complex with murine mast cell tryptases and heparin. PMID: 21566135
  • negative correlation between TSG-6 expression levels and severity of capsular contracture (CC) suggests a possible protective role for TSG-6 in the context of CC formation, and this may have a clinically relevant role in prevention of breast CC PMID: 21239672
  • TSG-6/HC2 link heavy chains randomly on the chondroitin sulfate chain of bikunin, in contrast to the ordered attachment observed during the biosynthesis. PMID: 20463016
  • polymorphism in osteoarthritis; chromosome location given here (chromosome 2) PMID: 11854277
  • TSG-6 is a multifunctional protein associated with inflammation [review] PMID: 12692188
  • Tumor necrosis factor, alpha-induced protein 6 activates an anti-inflammatory agent- inter-alpha-inhibitor[review]. PMID: 14515153
  • The TSG-6 and I alpha I interaction promotes a transesterification cleaving the protein-glycosaminoglycan-protein (PGP) cross-link. PMID: 15653696
  • This TSG-6 model reveals the residues most likely to be involved in stabilizing the inter-Link module interface, which are highly conserved across the Link protein/ chondroitin sulfate proteoglycan subfamily. PMID: 15718240
  • TSG-6 treatment also resulted in a rapid increase in cyclooxygenase-2 (COX-2) mRNA levels, suggesting that TSG-6 up-regulates COX-2 gene expression PMID: 15809059
  • TSG-6 acts as cofactor and catalyst in the production of IalphaI heavy chain x hyaluronan complexes PMID: 15840581
  • heparin with the Link module significantly increases the anti-plasmin activity of the TSG-6.IalphaI complex; TSG-6 is likely to contribute to matrix remodeling, at least in part, through down-regulation of the protease network PMID: 15917224
  • The tumor necrosis factor alpha induced protein 6 is a potential marker that could be used for early diagnosis and prognosis of cancerous or precancerous lesions. PMID: 16467113
  • hyaluronan synthase 2 and TSG-6 have roles in hyaluronan distribution and function in proximal tubular epithelial cells PMID: 16687630
  • Evidence is cited in this review that TSG-6 can regulate the expression of various molecules that have important roles in the control of inflammation. PMID: 16709183
  • TSG-6 is down-regulated during osteoblastic differentiation, and suppressed osteoblastic differentiation induced by both BMP-2 and osteogenic differentiation medium. PMID: 16771708
  • TSG-6 may play an important protective role in bronchial epithelium by increasing the antiprotease screen on the airway lumen. PMID: 16873769
  • Crystallography data on the free protein, and (15)N NMR relaxation data for the uncomplexed and HA(8)-bound forms of Link_TSG6, is presented. PMID: 17585936
  • TSG-6 is a high affinity ligand that can mediate fibronectin interactions with other matrix components and modulate some interactions of fibronectin with cells PMID: 18042364
  • 3 genes were upregulated in patients with chronic EBV infection: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5; they may be associated with the inflammatory reaction or with cell proliferation. PMID: 18260761
  • The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2. PMID: 18448433
  • TSG-6 has dual roles in bone remodeling: it inhibits RANKL-induced bone erosion in inflammatory diseases, and its interactions with BMP-2 and RANKL help to balance mineralization by osteoblasts and bone resorption by osteoclasts PMID: 18586671
  • TSG-6 transfers proteins between glycosaminoglycans via a Ser28-mediated covalent catalytic mechanism PMID: 18820257
  • myelomonocytic cells and MoDC are a major source of TSG-6 and that PTX3 and TSG-6 are coregulated PMID: 19389798

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    Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

    Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

    Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

    Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

    To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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