Recombinant Human TREM-1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4640
Recombinant Human TREM-1 Protein (His Tag)

Recombinant Human TREM-1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4640
Catalog No.: BLPSN-4640

Product Overview

Tag His
Host Species Human
Accession NP_061113.1
Synonym CD354, TREM-1
Background TREM1 (triggering receptor expressed on myeloid cells) is a type I transmembrane protein with a single Ig-like domain, and is selectively expressed on blood neutrophils and a subset of monocytes. As a member of the growing family of receptors related to NK cell receptors, TREM1 activates downstream signaling events with the help of an adapter protein called DAP12. Expression of TREM1 is up-regulated by bacterial LPS, a ligand for TLR4, as well as lipoteichoic acid. Although its natural ligand has not been identified, engagement of TREM1 with agonist mAbs triggers secretion of the proinflammatory cytokines TNF-alpha and IL-1beta, as well as chemokines such as IL-8 and monocyte chemoattractant protein (MCP)-1. Intracellularly, TREM1 induces Ca2+ mobilization and tyrosine phosphorylation of extracellular signal-related kinase 1 (ERK1), ERK2 and phospholipase C-γ. In an animal model of LPS-induced septic shock, blockade of TREM1 signaling inhibited hyperresponsiveness and death. Thus, it has been demonstrated that TREM1 performs a critical function in immune responses involved in host defense against microbial challenges, and is suggested to be a potential therapeutic target for septic shock.
Description A DNA sequence encoding the extracellular domain (Met 1-Arg 200) of human TREM1 (NP_061113.1) was fused with the a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Ala 21
AA Sequence Met 1-Arg 200
Molecular Weight The secreted recombinant human TREM1 consists of 191 a.a. and has a predicted molecular mass of 21.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTREM1 is approximately 38-42 kDa due to glycosylation.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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