Recombinant Human Transglutaminase 3 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4635
Recombinant Human Transglutaminase 3 Protein (His Tag)

Recombinant Human Transglutaminase 3 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4635
Catalog No.: BLPSN-4635

Product Overview

Tag His
Host Species Human
Accession Q08188
Synonym TGE
Background Transglutaminases (TGase) are a family of calcium-dependent acyl-transfer enzymes ubiquitously expressed in mammalian cells and responsible for catalyzing covalent cross-links between proteins or peptides. Transglutaminase 3 (TGM3) is a member of a family of Ca2+-dependent enzymes that catalyze covalent cross-linking reactions between proteins or peptides. TGM3 isoform is widely expressed and is important for epithelial barrier formation. It is a zymogen, requiring proteolysis for activity. Calcium-activated TGM3 can bind, hydrolyze, and is inhibited by GTP, despite lacking structural homology with other GTP binding proteins. TGM3 displays a diffuse cytoplasmic distribution in vitro consistent with its proposed role in the early phase of cornified cell envelope assembly in the cytoplasm. TGM3-driven specific isopeptide bonds between intermediate filaments and KAPs participate to the progressive scaffolding of the hair shaft. Additionally, TGM3 may be a novel prognostic biomarker for esophageal squamous cell carcinoma (ESCC).
Description A DNA sequence encoding the human TGM3 (Q08188) (Ala 2-Glu 693) was expressed, with a His tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal His
AA Sequence Ala 2-Glu 693
Molecular Weight The recombinant human TGM3 consists of 710 a.a. and predicts a molecular mass of 78.8 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH.The specific activity is > 450 pmoles/min/ug.
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% gly.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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