Recombinant Human Transglutaminase 3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4635
Recombinant Human Transglutaminase 3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4635
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | Q08188 |
Synonym | TGE |
Background | Transglutaminases (TGase) are a family of calcium-dependent acyl-transfer enzymes ubiquitously expressed in mammalian cells and responsible for catalyzing covalent cross-links between proteins or peptides. Transglutaminase 3 (TGM3) is a member of a family of Ca2+-dependent enzymes that catalyze covalent cross-linking reactions between proteins or peptides. TGM3 isoform is widely expressed and is important for epithelial barrier formation. It is a zymogen, requiring proteolysis for activity. Calcium-activated TGM3 can bind, hydrolyze, and is inhibited by GTP, despite lacking structural homology with other GTP binding proteins. TGM3 displays a diffuse cytoplasmic distribution in vitro consistent with its proposed role in the early phase of cornified cell envelope assembly in the cytoplasm. TGM3-driven specific isopeptide bonds between intermediate filaments and KAPs participate to the progressive scaffolding of the hair shaft. Additionally, TGM3 may be a novel prognostic biomarker for esophageal squamous cell carcinoma (ESCC). |
Description | A DNA sequence encoding the human TGM3 (Q08188) (Ala 2-Glu 693) was expressed, with a His tag at the N-terminus. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | His |
AA Sequence | Ala 2-Glu 693 |
Molecular Weight | The recombinant human TGM3 consists of 710 a.a. and predicts a molecular mass of 78.8 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH.The specific activity is > 450 pmoles/min/ug. |
Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% gly. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |