| Catalog Number | BLE-0042P |
|---|---|
| Target Name | Transforming growth factor beta 3 |
| Target Symbol | TGFB3 |
| Species | Human |
| Source | Recombinant |
| Protein Expression Range | Ala 301-Ser 412 |
| Molecular Weight | 13 kDa |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Tag | Tag-Free |
| Product Grade | GMP-Grade |
| Active | Yes |
| Endotoxin Level | ≤10 EU/mg by the LAL method. |
| Endotoxin Classification | Ultra-Low Endotoxin |
| Bioactivity | Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. |
|---|
| Formulation | Lyophilized |
|---|---|
| Buffer | Lyophilized from a 0.22 μm-filtered solution containing 100 mM Glycine, 150 mM NaCl, 5% Mannitol and 0.01% Tween 80, pH4.0. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution; Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Accession | P10600 |
|---|---|
| Alternative Names | Transforming growth factor beta-3; TGFB3; TGF-beta-3; Latency-associated peptide; LAP |
Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.
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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.
Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.
Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.
Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.
To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.
Inquire the Recombinant Human Transforming Growth Factor Beta 3 (TGFB3) Protein, Tag-Free, Active, GMP-Grade, Ultra-Low Endotoxin by filling out the form below. We’ll get back to you within 24 hours with a quote.
![SDS-PAGE analysis of Human NDUFV1 (Nadh Dehydrogenase [Ubiquinone] Flavoprotein 1, Mitochondrial) - Recombinant Protein, CAT# BLT-08321P, showing >90% purity under 15% SDS-PAGE (Reduced)](http://www.betalifesci.com/cdn/shop/files/1ihmbkjr21vdmc6j_{width}x.jpg?v=1753363793)
