Recombinant Human tPA Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-4611
Recombinant Human tPA Protein (Fc Tag)

Recombinant Human tPA Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-4611
Catalog No.: BLPSN-4611

Product Overview

Tag Fc
Host Species Human
Accession NP_000921.1
Synonym T-PA, TPA
Background Tissue plasminogen activator (abbreviated tPA or PLAT), is traditionally viewed as a simple serine protease whose main function is to convert plasminogen into biologically active plasmin. As a protease, tPA plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. tPA is synthesized and secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Proteolytic cleavage at the C-terminal side of Arg275 generates the enzyme composed of two subunits, designated as alpha and beta chains which are held together by a single disulfide bond. Unlike the other members of the chymotrypsin family, tPA has one particular distinction in that the catalytic efficiency of the single-chain enzyme is only slightly lower than that of the proteolytically cleaved form and is therefore not a true zymogen. tPA is found not only in the blood, where its primary function is as a thrombolytic enzyme, but also in the central nervous system (CNS). It participats in a number of physiological and pathological events in the CNS, as well as the role of neuroserpin as the natural regulator of tPA's activity in these processes. Increased or decreased activity of tPA leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, as a cytokine, tPA plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as a fibrogenic cytokine, it promotes the progression of kidney diseases.
Description A DNA sequence encoding the alpha chain (Tyr 37-Arg 310) of mature human tPA (NP_000921.1) was expressed with the fused Fc region of human IgG1 at the N-terminus.
Source HEK293
Predicted N Terminal Glu 20
AA Sequence Tyr 37-Arg 310
Molecular Weight The recombinant human Fc/tPAalpha is a disulfide-linked homodimeric protein. The reduced monomer consists of 512 a.a. and has a predicted molecular mass of 57.5 KDa. As a result of glycosylation, the apparent molecular mass of rh Fc/tPAalpha monomer is approximately 59 KDa in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 50mM NaAc, 150mM NaCl, 5mM CaCl2, 10% glycerol, PH 5.5.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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