Recombinant Human TNF-alpha Protein, Animal-Free, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0046UL
Recombinant Human TNF-alpha (2 μg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at about 15 kDa and purity >98%.
Recombinant Human TNF-alpha (2 μg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at about 15 kDa and purity >98%.

Recombinant Human TNF-alpha Protein, Animal-Free, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0046UL
/
Size

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Product Overview

Product Name Recombinant Human TNF-alpha Protein, Animal-Free
Target Symbol TNF
Alternative Names Tumor Necrosis Factor alpha, TNF, TNFA, APC1, TNFSF2, Tumor Necrosis Factor Ligand Superfamily Member 2, Differentiation Inducing Factor, DIF, Cachectin
Accession Number P01375.1
Expression System E. coli using an animal component-free system.
Molecular Weight 17.4 kDa (monomer, predicted)
Sequence Val77-Leu233
Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by a cell proliferation assay using mouse L-929 cells treated with Actinomycin D. The ED50 for this effect is typically <40 pg/mL.
Formulation Lyophilized from sterile phosphate buffer with Trehalose, pH 8.0
Endotoxin Level <0.001 EU per 1 μg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. Optionally, use PBS with 0.1% BSA.
Target Function Tumor Necrosis Factor-alpha (TNF-alpha) was initially associated as a proinflammatory cytokine expressed by macrophages. However, TNF-alpha also regulates apoptosis, tumor cell death, lipid metabolism and cell differentiation of T cells, B cells and NK cells. There are 2 receptors for TNF-alpha, a ubiquitously expressed TNFR1 (CD120a) homotrimer containing an apoptotic pathway trigger and the TNFR2 (CD120b) homotrimer that is commonly restricted to epithelial, endothelial and specific immune cells.
Tissue Specificity TNF-alpha expression has been identified in adipose tissue, microglia, fibroblasts, endothelia tissue, and epithelial tissue.
Cellular Localization Membrane-bound and cleaved soluble protein forms
Involvement In Disease Dysregulation of TNF-alpha is implicated in rheumatoid arthritis, inflammatory bowel disease (IBD), psoriasis, ankylosing spondylitis, systemic lupus erythematosus (SLE), systemic sclerosis (SSc), multiple sclerosis (MS) and insulin resistance in type 2 diabetes

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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