Recombinant Human TIM-1 Protein

Beta LifeScience SKU/CAT #: BLPSN-4503

Recombinant Human TIM-1 Protein

Beta LifeScience SKU/CAT #: BLPSN-4503
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Product Overview

Tag N/A
Host Species Human
Accession AAC39862.1
Synonym CD365, HACVR, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1
Background HAV cellular receptor 1 (HAVCR1), also known as Kidney injury molecule 1 (KIM-1) and T cell immunoglobulinmucin 1 (TIM-1), is a type â-€¦ integral membrane glycoprotein. KIM-1 protein is widely expressed with highest levels in kidney and testis. It has been shown to play a major role as a human susceptibility gene for asthma, allergy and autoimmunity. IgA1lambda is a specific ligand of KIM-1 protein and that their association has a synergistic effect in virus-receptor interactions. KIM-1 involves in the pathogenesis of acute kidney injury. It had been confirmed that KIM-1 is a human urinary renal dysfunction biomarker. Moreover, KIM-1 protein is a novel regulatory molecule of flow-induced calcium signaling.
Description A DNA sequence encoding the mature form of human KIM1 extracellular domain (AAC39862.1) (Ser 21-Gly 290) with a C-terminal His tag was expressed and purified.
Source HEK293
Predicted N Terminal Ser 21
AA Sequence Ser 21-Gly 290
Molecular Weight The secreted recombinant human KIM1 comprises 278 a.a. and predicts a molecular mass of 30 kDa. It migrates with the molecular weight in the range of 90-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair.; (Microbial infection) Acts as a receptor for Hepatitis A virus.; (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane. Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP.; (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane.; (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E.
Subcellular Location Membrane; Single-pass type I membrane protein.
Protein Families Immunoglobulin superfamily, TIM family
Database References
Tissue Specificity Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.

Gene Functions References

  1. neither the inflammatory biomarker calprotectin, nor the tubular biomarkers NGAL and KIM-1, provide robust prognostic information on the loss of renal function in a heterogeneous CKD population. All of them, however, are candidate prognostic biomarkers in primarily inflammatory renal diseases. PMID: 30078006
  2. High KIM1 expression is associated with Clear Cell Renal Cell Carcinoma. PMID: 29295730
  3. Human oligodendrocytes undergo apoptosis when exposed to Sema4A and take up H-ferritin for meeting iron requirements and that these functions are mediated via the Tim-1 receptor. H-ferritin can block Sema4A-mediated cytotoxicity. Sema4A is detectable in the CSF of multiple sclerosis patients and HIV-seropositive persons and can induce oligodendrocyte cell death. PMID: 29457657
  4. Elevated plasma NT-proBNP and KIM-1 in patients with stage 5 chronic kidney disease (CKD5) undergoing maintenance hemodialysis (HD) and HF (+) (heart failure)CKD5-HD patients suggest that natriuretic peptides and KIM-1 may contribute to the pathogenesis of HF in CKD5-HD patients. PMID: 28990414
  5. These findings suggest that the TIMD4-HAVCR1 variants may be the genetic risk factors for coronary heart disease and ischemic strok PMID: 29208769
  6. These studies provide evidence that, in the absence of a functional alpha-dystroglycan, TIM-1 mediates the entry of Lassa virus pseudoviral particles through interactions of virions with the IgV PtdSer-binding pocket of TIM-1. PMID: 29875238
  7. total flavone of Desmodium styracifolium inhibited HK-2 cell apoptosis and autophagy by regulating KIM-1 via p38/MAPK pathway. PMID: 29071538
  8. Polymorphism +4259A>C in exon 3 of the TIM-3 gene is associated with susceptibility to multiple sclerosis but polymorphism -1637C>T in the promoter region of TIM-1 is not. PMID: 29141799
  9. High KIM1 expression is associated with decreased proximal tubular epithelial cell function. PMID: 29045706
  10. findings show that TIM1 is not an essential cellular receptor for hepatitis A virus entry into cultured cells PMID: 28874468
  11. Urinary excretion of KIM-1 correlates with blood lead levels better than other traditional renal injury biomarkers, including N-acetyl-beta-(D)-glucosaminidase, alpha1-microglobulin, and beta2-microglobulin. PMID: 27966578
  12. promising results were obtained for circulating KIM-1 prediction of renal outcomes in type 1 diabetes--{REVIEW} PMID: 27771693
  13. data show that in addition to the phospholipid-binding function of HAVCR1, the enhancement of HCV infection involves other determinants in the IgV domain of HAVCR1. These findings expand the repertoire of molecules that HCV uses for cell entry, adding to the already complex mechanism of HCV infection and pathogenesis. PMID: 29321304
  14. Review/Meta-analysis: suggest that TIM-1-416G[C single nucleotide polymorphism is associated with the risk of asthma for the Asian ethnicity in the codominant (G/G vs. G/C) and dominant (G/G vs. G/C + C/C) genetic models. PMID: 28434117
  15. Urinary NGAL is a good predictor of mortality in established pediatric acute kidney injury (AKI) of heterogeneous etiology and outperforms calprotectin and KIM-1. Urinary calprotectin and KIM-1 predict renal replacement therapy requirement in pediatric AKI. PMID: 28409285
  16. A panel consisting of IGFBP1, KIM1, GCLC and GSTM1 genes could be used in combination for early screening of CKDu, whereas these genes in addition with FN1, IGFBP3 and KLK1 could be used to monitor progression of CKDu. The regulation of these genes has to be studied on larger populations to validate their efficiency for further clinical use. PMID: 28103909
  17. Quantification of TIM-3 and KIM-1 mRNA expressions, along with KIM-1 protein measurements in urine and blood could be employed as promising tools for noninvasive diagnosis of allograft dysfunction. PMID: 28757398
  18. Report detection of drug-induced acute kidney injury in humans by combining the sensitivity of urinary KIM-1 along with urinary miR-21, -200c, and -423. PMID: 27122240
  19. measurement of urinary and renal KIM-1 level may be helpful to evaluate severity of renal pathological damage and prognosis in adult Henoch-Schonlein purpura patients with nephritis PMID: 29122357
  20. Urine KIM-1 level in acute kidney injury patients was significantly higher than that in the healthy controls. PMID: 28212617
  21. PCNSL is characterized by frequent Tim-1 expression, and its soluble form in CSF may become a useful biomarker for PCNSL. PMID: 27709813
  22. TIM-1 is a unique marker for the identification of a human IL-10(+) Breg subpopulation which is partially superimposed with transitional B cells PMID: 28103916
  23. in the current meta-analysis, based on ten prospective studies involving 29366 participants, we evaluated the role of urinary tubular injury markers (NGAL, KIM-1 and NAG) in predicting clinical outcomes including CKD stage 3, end stage renal disease and mortality. PMID: 27907168
  24. Urinary KIM-1 levels in the acute kidney injury (AKI) preterm infant group were not significantly higher than in no-AKI group on day of life 1, 3 and 7. PMID: 27020372
  25. We conclude that KIM-1 might serve as a sensitive biomarker to screen children for kidney damage induced by environmental toxic agents, such as chromium and arsenic PMID: 27431456
  26. These results suggest that a 6-amino acid deletion polymorphism in the mucin domain of TIM-1 protects from HIV-1 infection with a recessive effect. PMID: 27652980
  27. The present study shows that serum and urine levels of NGAL and KIM-1 are higher in patients with acute kidney injury than in those without acute kidney injury, and that serum NGAL and the presence of chronic kidney disease are significant predictors of acute kidney injury in scrub typhus. PMID: 28419138
  28. KIM-1 increases albumin endocytosis in renal tubule epithelial cells. PMID: 26332568
  29. preliminary data indicates that KIM-1 expression may be associated with stage in Wilms Tumor PMID: 27562389
  30. Urinary KIM-1 and NAG levels were positively correlated. PMID: 27272162
  31. TLR2, Tim1, Tim3, TNF-alpha, IgE and EOS play a role in Mycoplasma pneumoniae Pneumonia-related wheezing in children. PMID: 27185658
  32. The results of our study showed that there is no significant association between TIM-1 5383-5397ins/del and Multiple sclerosis (MS) (P = 0.38); however, the frequency of CT genotype of TIM-3 -1541C>T in patient group was significantly higher than the control group, and there was a significant association between CT genotype and MS PMID: 27091308
  33. Study emphasizes a strong association of the tubular biomarker KIM-1 with chronic kidney disease progression in chronic heart failure and suggests usefulness as cardiorenal marker. PMID: 26422793
  34. KIM-1 expression also enhances ERK MAPK activation, and the modulatory effect of KIM-1 on cellular repair process is likely mediated via ERK MAPK signaling pathway. PMID: 27084535
  35. KIM-1 may mediate high glucose-induced autophagy and apoptosis in renal tubular epithelial cells. PMID: 27310004
  36. Study suggests that TIM1 -416G>C and -1454G>A single nucleotide polymorphisms are associated with dilated cardiomyopathy susceptibility and prognosis in this Han Chinese population. PMID: 27818477
  37. elevated baseline plasma KIM-1 strongly associated with risk of early progressive renal decline in type 1 diabetes PMID: 26509588
  38. Urinary KIM-1 levels increased significantly in patients with contrast-induced nephropathy. PMID: 26474236
  39. Data show that kidney injury molecule (KIM)-1 and neutrophil gelatinase-associated lipocalin (NGAL), were significantly elevated in welding workers post-exposure. PMID: 26673824
  40. High NGAL and KIM-1 levels may indicate early diabetic kidney injury; however, we did not observe any relationship between these markers and diabetic indices PMID: 26393328
  41. Kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin excretion rates correlated with excretion rates of other tubular damage markers and predicted outcome in patients with idiopathic membranous nephropathy PMID: 25762211
  42. The aim of this review article is to summarize the literature data concerning KIM-1 as a potential novel marker in the early diagnosis and prediction of clinical outcome of certain cardiovascular diseases. PMID: 26697493
  43. Activation of TIM1 by exposing the cells to TIM4 significantly increased the frequency of apoptotic colon cancer cells. The expression of FasL was increased in the cancer cells after treating by TIM4. PMID: 26921445
  44. Urine KIM1 level was higher in kidney stone patients before lithotripsy than in healthy controls. PMID: 26407192
  45. Urinary levels of NGAL are more sensitive than uKIM-1 and uL-FABP levels in predicting renal scarring in vesicoureteral reflux. PMID: 26324091
  46. TIM-1 ligands increase the amount of cell surface protein, preventing its traffic to the immune synapse PMID: 26332704
  47. Urinary KIM-1 measured 24 h after the start of drug infusion has the potential to detect early AKI in pediatric patients treated with MTX or platinum-class drugs PMID: 26248472
  48. Urinary KIM-1 is a significant predictor of death in extremely low-birth-weight neonates. PMID: 26107391
  49. Urinary KIM1 is significantly higher in patients with clear cell renal carcinoma than in controls. Urinary KIM1 decreases after nephrectomy in clear cell renal carcinoma patients. PMID: 27295912
  50. decreased serum TIM-1 and TIM-3 levels and association of TIM-3 with nephritis suggest their possible role in the development and pathogenesis of systemic lupus erythematosus. PMID: 27049082


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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