Recombinant Human Thrombopoietin/TPO Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0027
Recombinant Human Thrombopoietin/ TPO Protein (2 ug/lane) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie® Blue showing a single band at 20 kDa and 18 kDa, respectively, and purity greater than 95%.
Recombinant Human Thrombopoietin/ TPO Protein (2 ug/lane) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie® Blue showing a single band at 20 kDa and 18 kDa, respectively, and purity greater than 95%.

Recombinant Human Thrombopoietin/TPO Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0027
/
Size

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Product Overview

Product Name Recombinant Human Thrombopoietin/TPO Protein
Target Symbol THPO
Alternative Names Thrombopoietin, THPO, TPO, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth And Development Factor, Megakaryocyte Colony-Stimulating Factor, Thrombopoietin Nirs, C-Mpl Ligand, MPLLG, MGDF, THCYT1, CAMT2, MKCSF
Accession Number P40225
Expression System CHO cell line
Molecular Weight 18.7 kDa (monomer, predicted), 20 kDa and 18 kDa (glycosylated, observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively)
Sequence Ser22-Leu195
Purity >95% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity Determined by a cell proliferation assay using MO7e human cells. The ED50 for this effect is typically <10 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.1 EU/1ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Thrombopoietin (TPO) plays an important role in the proliferation and maturation of megakaryocytes and their progenitors in regulating platelet production. TPO has a role in promoting the survival, self-renewal, and expansion of hematopoietic stem cells, along with primitive multilineage progenitor cells. This has led to further interest in applications for in vitro and ex vivo maintenance, expansion and differentiation of hematopoietic stem cells. TPO is the ligand for c-Mpl (CD110).
Tissue Specificity TPO is primarily synthesized in the liver, and to lesser degrees in the kidneys, bone marrow and spleen.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of TPO is associated with idiopathic thrombocytopenic purpura (ITP), aplastic anemia, myelodysplastic syndromes (MDS), thrombocytosis, hepatitis, cirrhosis, and chemotherapy-induced thrombocytopenia.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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