Recombinant Human Thrombomodulin Protein (C-6His)

Name: Recombinant Human Thrombomodulin Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1651NP
Availability: InStock

BL-1651NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Thrombomodulin is produced by our Mammalian expression system and the target gene encoding Ala19-Ser515 is expressed with a 6His tag at the C-terminus.
Accession	P07204
Synonym	Thrombomodulin; THBD; TM; Fetomodulin; CD141; THRM
Gene Background	Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Human Thrombomodulin/THBD predicts a signal peptide and a mature chain that consists of following domains: C-type lectin, EGF-like, transmembrane and cytoplasmic. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis. THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer. For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis.
Molecular Mass	52.9 KDa
Apmol Mass	72 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Database References	HGNC: 11784 OMIM: 188040 KEGG: hsa:7056 STRING: 9606.ENSP00000366307 UniGene: PMID: 30008974 SETD1A contributes to retinoic acid -induced thrombomodulin expression in vascular endothelial cells by modulating the activity and expression of KLF4. PMID: 29940355 we for the first time demonstrated that TM binds to GPR15 via its EGF-like domain and exerts angiogenesis and cytoprotective function in vascular ECs. PMID: 28386128 Bioinformatics analysis and screening of controls strongly suggested that the THBD-p.Trp153Gly mutation might be related to RPL aetiology. PMID: 29195508 Taken together, our findings suggest that TM-PKCdelta interaction may contribute to cardiovascular disorders by affecting monocye differentiation, which may develop future therapeutic applications. PMID: 27910925 A heterozygous variant displaying autosomal dominant inheritance (c.1611 C>A) was found in the gene THBD which encodes the glycoprotein thrombomodulin. This sequence change results in a stop codon (p.Cys537Stop) and truncation of the protein PMID: 28267383 Lys 42, Lys 43, Lys 44 and Arg 12 are critical for the interaction of TAFI with the thrombin-thrombomodulin complex, which modulates its antifibrinolytic potential. PMID: 28640323 Ligation of anti-HLA class I and II antibodies produces different effects on the endothelial expression of TBM and on serum levels of TBM in transplant recipients. PMID: 28239987 Fibrinogen gamma acts as thrombomodulin II. (Review) PMID: 27784620 Thrombomodulin (TM) promotes angiogenesis by enhancing cell adhesion, migration, and FAK activation through interaction with fibronectin. PMID: 27602495 The elevation of serum thrombomodulin (sTM)level suggests that endothelial damage occurs in Abdominal Aortic Aneurysm pathogenesis. PMID: 28473982 This population-based cohort study within the ARIC study did not replicate the Hernandez et al. finding that carrying the minor allele of 3 THBD SNPs doubles the risk of venous thromboembolism in African Americans. In fact, the HRs of VTE among carriers of the minor allele were <1. HRs were similar for white subjects. A strand-flip did not explain the discrepancies. PMID: 28619983 these results suggest a novel function for thrombomodulin as an adhesion molecule in monocytes, where it enhances cell adhesion by binding Ley, leading to beta2 integrin activation via p38 MAPK PMID: 27808085 TM, especially TME45, maintains vascular integrity, at least in part, via Src signaling. PMID: 27643869 The present study found that the fifth epidermal growth factor-like domain of thrombomodulin (TME5) possesses the cytoprotective function in association with an increase in levels of anti-apoptotic myeloid cell leukemia-1 protein in an activated protein C-independent PMID: 27427915 Case Report: CD141+ myeloid dentritic cell differentiation of a juvenile myelomonocytic leukemia. PMID: 28414089 the effect of thromobomodulin c.1418C > T polymorphism on the pathogenesis of venous thrombosis PMID: 28710034 The finding of a previously unrecognized fibrinolytic phenotype indicates that bleeding in Thrombomodulin-associated coagulopathy has a complex pathogenesis and highlights the pivotal role of TM as a regulator of hemostasis. PMID: 27436851 TM mediates cell proliferation and migration via the Epithelial-To-Mesenchymal Transition (EMT) biomarkercyclooxygenase (COX)-2 PMID: 27512995 the whole THBD gene sequenced in patients with recurrent venous thromboembolism (VTE); found 8 polymorphisms in the THBD gene in Swedish population; none of these polymorphisms was significantly associated with the risk of VTE recurrence; results indicate that THBD polymorphisms may not be a risk factor for VTE recurrence PMID: 28049360 CORM-2 protects human umbilical vein endothelial cells from lipopolysaccharide-induced injury, by way of suppressing NF-kappaB activity, which downregulates TM and EPCR mRNAs. It also decreases MMP-2 expression and prevents the shedding of TM and EPCR from the surface of endothelial cells, so as to preserve their protective effect. PMID: 28538400 The results demonstrate that the LFA-1 and Mac-1 integrins on leukocytes bind to thrombomodulin (TM), thereby establishing the molecular and structural basis underlying LFA-1 and Mac-1 integrin interaction with TM on endothelial cells. PMID: 27055590 Human thrombomodulin transgenic aortic endothelial cells are less sensitive to activation by either HMGB1 or hTNFalpha, an effect that appears to be dependent on the lectin-like domain of TBM. PMID: 27077599 In placenta of patients with preeclampsia, we detected abnormal expression of F3 and THBD with increased protein and mRNA levels. The role of these molecules in the pathogenesis of this disease and in alterations of hemostatic and histopathological aspects of placentas need further studying. PMID: 27002259 recombinant TM (Solulin) can protect the intestine from toxicity in a clinically relevant rat model PMID: 27459702 TM up-regulated E-cadherin but down-regulated N-cadherin expression, resulting in reversal of epithelial-mesenchymal transition (EMT) in the lung cancer cells. PMID: 27223053 High serum thrombomodulin expression is associated with non-alcoholic fatty liver disease. PMID: 26959535 results do not suggest a predictive role for THBD c.1418C>T polymorphism in VTE recurrence PMID: 26743062 R12 is a critical residue for the activation of TAFI by thrombin-thrombomodulin PMID: 26816270 Study detected a statistically significant positive correlation between expanded disability status scale scores and thrombomodulin levels (p<0.01) and a 10% positive correlation between expanded disability status scale scores and APC levels in multiple sclerosis patients PMID: 27456888 Case Report: thrombotic microangiopathy with mutations in complement factor I and thrombomodulin. PMID: 26613809 increased plasma TM levels and serum hs-CRP levels in cerebral infarction (CI) patients were associated with the development of CI in Asians. PMID: 26133301 evidence of association between the -33G/A polymorphism in the TM gene and the risk of myocardial infarction in Asians; the Ala455Val variant was not associated with atherosclerotic risk [meta-analysis] PMID: 26888356 Decreased thrombomodulin expression in preeclampsia may play a role in placental dysfunction in preeclampsia and is possibly caused by an angiogenic imbalance. Hypertension and obesity are associated with thrombomodulin downregulation. PMID: 26891741 The presence of THBD proximal promoter polymorphisms do not explain variations in levels of serum and cell-expressed THBD in premature acute coronary syndrome patients in Bahrain. PMID: 26226255 the functional relevance of the rs3176123 variation and indicate that higher thrombomodulin expression by individuals with the 2729C allele likely accounts for their decreased risk for acute GVHD development and subsequent mortality. PMID: 26246110 The lack of any association between the sTM levels and genetic variants in ARDS suggests that the increased levels of sTM may reflect severity of endothelial damage rather than genetic heterogeneity. PMID: 25643902 Identified Nur77/Nor1 as novel regulators of thrombomodulin expression and function in vascular endothelial cells. PMID: 26634653 The results of this study supported the association of the epistatic interactions of ALOX5AP, THBD, and KNG1 and present novel evidence for the main effect of KNG1 gene on IS susceptibility. PMID: 26159646 The EGF5, 6 domains of thrombomodulin appear to be the major domains for down-regulating the complement system rather than the lectin-like domain during xenogenic stimuli. PMID: 26179123 A minimal TM fragment consisting of the fourth, fifth, and most of the sixth EGF-like domain (TM456m) that has been prepared has much improved solubility, thrombin binding capacity, and anticoagulant activity versus those of previous TM456 constructs. PMID: 26468766 Data indicate that blood dendritic cell antigen 3 BDCA3(+) and C-type lectin domain family 9, member A CLEC9A(+) dendritic cells (DC) are of major importance in the induction of anti-viral and anti-tumor immunity. PMID: 24910448 Recombinant thrombomodulin does not impair neutrophil functions. PMID: 25214376 levels of protein C and soluble thrombomodulin in critically ill patients with acute kidney injury PMID: 25790110 Membrane-bound TM in macrophages plays an essential role in the development of abdominal aortic aneurysms by enhancing proinflammatory mediator elaboration, macrophage recruitment, and oxidative stress. PMID: 26338301 Cyclic strain strongly downregulated TM expression in a p38- and receptor tyrosine kinase-dependent manner in aortic endothelial cells. PMID: 25238231 kinetics of the interaction between serine/threonine-rich domain of thrombomodulin (rTMD23) and FGFR1 were analysed in umbilical vein endothelial cells PMID: 25388665 Thrombomodulin is differentially regulated within cultured brain microvascular endothelial cells by cytokines and shear stress. PMID: 25250518 Review/Meta-analysis: TM -33G/A and Ala455Val polymorphisms were risk factors for coronary artery disease. PMID: 25144670 function and regulation of BDCA3 expression and IFN-lambda production by dendritic cells PMID: 25616220

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human Thrombomodulin Protein (C-6His)

Recombinant Human Thrombomodulin Protein (C-6His)

Product Overview

Target Details

FAQs