Recombinant Human TALDO1 Protein (C-6His)

Name: Recombinant Human TALDO1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1387NP
Availability: InStock

BL-1387NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Transaldolase is produced by our Mammalian expression system and the target gene encoding Met1-Lys337 is expressed with a 6His tag at the C-terminus.
Accession	P37837
Synonym	Transaldolase; TALDO1; TAL; TALDO; TALDOR
Gene Background	Transaldolase (TALDO1) belongs to the transaldolase family of Type 1 subfamily. TALDO1 is expressed selectively in oligodendrocytes of the brain. TALDO1 is a key enzyme of the nonoxidative pentose phosphate pathway providing ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This pathway can also maintain glutathione at a reduced state and thus protect sulfhydryl groups and cellular integrity from oxygen radicals. TALDO1 deficiency results in telangiectases of the skin, hepatosplenomegaly and enlarged clitoris.
Molecular Mass	38.6 KDa
Apmol Mass	38 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Glycerol, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.
Subcellular Location	Cytoplasm.
Protein Families	Transaldolase family, Type 1 subfamily
Database References	HGNC: 11559 OMIM: 602063 KEGG: hsa:6888 STRING: 9606.ENSP00000321259 UniGene: PMID: 27703206 The above findings support the premise that biallelic mutations in TALDO1 are responsible for transaldolase deficiency and confirm the broad phenotypic variability of this condition, even with the same genotype. PMID: 25388407 This study expands the clinical definition of transaldolase deficiency, and adds to its allelic heterogeneity. PMID: 23315216 Data suggest that exchange reactions during gluconeogenesis catalyzed by transaldolase and triose-phosphate isomerase do not differ between subjects with type 2 diabetes and control subjects under fasting or hyperglycemic conditions. PMID: 23736541 renal phenotype of patients with transaldolase deficiency PMID: 22510381 these data provide strong experimental evidence that transaldolase exchange occurs in humans, resulting in an overestimate of gluconeogenesis PMID: 21062960 Data show that erythronic acid was identified as a major abnormal metabolite in all patients and in knock-out TALDO mice implicating an as yet unknown biochemical pathway in this disease. PMID: 20600873 granzyme B-cleaved transaldolase-specific T cell-mediated cytotoxicity may contribute to the progressive destruction of oligodendrocytes in patients with multiple sclerosis PMID: 20194725 transaldolase is regulated by ZNF143 in a tissue-specific manner PMID: 14702349 TALase undergoes anterograde trafficking in neutrophils from nonpregnant individuals, whereas retrograde trafficking is found during pregnancy PMID: 16092052 Mutation in the TALDO1 gene was found in patients with hydrops fetalis and neonatal multi-organ disease. PMID: 17095351 A patch of functionally important amino acid residues extends from serine-171 toward the catalytic site and is proposed as a novel ligand shuttling path connecting these specific sites to the enzyme's active site. PMID: 17503352 Transaldolase-deficient patients had significantly increased urinary heptoses, revealing novel urinary biomarkers for identification of the deficiency. PMID: 17603756 A new case of TALDO deficiency resulted in cirrhosis, rickets and deafness. PMID: 18331807 The present study identified the TAL deficiency as a modulator of mitochondrial homoeostasis, Ca(2+) fluxing and apoptosis. PMID: 18498245 analysis of enzymes TalB and Taldo1 in human and E. coli PMID: 18687684 genetic polymorphisms in Transaldolase 1 are associated with squamous cell carcinoma of the head and neck . PMID: 18805652

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Human TALDO1 Protein (C-6His)

Recombinant Human TALDO1 Protein (C-6His)

Product Overview

Target Details

FAQs