Recombinant Human Syndecan-4 (SDC4), Active

Name: Recombinant Human Syndecan-4 (SDC4), Active
Brand: Beta LifeScience
SKU: BLC-05681P
Availability: InStock

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Syndecan-4 (SDC4), Active is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 95% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The specific activity is determined by binding ability in a functional ELISA. Immobilized rHuSYND4 at 500 ng/ml (100 μl/well) can bind rHubFGF with a linear range of 0.1-10 ng/ml.
Uniprotkb	P31431
Target Symbol	SDC4
Synonyms	Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; Sdc4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4; Syndecan4
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	ESIRETEVID PQDLLEGRYF SGALPDDEDV VGPGQESDDF ELSGSGDLDD LEDSMIGPEV VHPLVPLDNH IPERAGSGSQ VPTEPKKLEE NEVIPKRISP VEESEDVSNK VSMSSTVQGS NIFERTE
Expression Range	19-145aa
Protein Length	Partial
Mol. Weight	13.9 kDa
Research Area	Signal Transduction
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Subcellular Location	[Isoform 1]: Membrane; Single-pass type I membrane protein. Secreted.; [Isoform 2]: Secreted.
Protein Families	Syndecan proteoglycan family
Database References	HGNC: 10661 OMIM: 600017 KEGG: hsa:6385 STRING: 9606.ENSP00000361818 UniGene: PMID: 30165731 Downregulation of SDC-4 inhibited FGF signaling through the blockade of ERK1/2 and PI3K/Akt/mTOR activation, thus suppressing cell proliferation and migration. PMID: 28703800 studies are required to show if syndecan-4 concentrations can be marker for prognosis assessment or disease progression PMID: 29232393 Data provide evidence that SDC4 plays important roles in normal physiology of intervertebral disc and cartilage through controlling growth factor signaling and matrix homeostasis. However, several studies to date clearly show that in diseased joints, SDC4 and inflammatory cytokines IL-1beta and TNF-alpha form a positive feedback loop, wherein they control each other's expression and/or activity. [review] PMID: 26796346 This study demonstrates that the shedding of synd4 from Endothelial progenitor cells (EPCs) plays a key role in advanced glycation end products-mediated dysfunction of EPC migration and homing. PMID: 27662820 the Ser179Glu mutant of SDC-4 binds strongly Tiam1, a Rac1-GEF reducing Rac1-GTP by 3-fold in MCF-7 breast adenocarcinoma cells. PMID: 29121646 Syndecan 4 is the biomarker independently distinguishing Heart Failure with preserved ejection fraction and Heart Failure with reduced ejection fraction. PMID: 27448535 The upregulation of syndecan-4 in the eutopic endometrium of endometriosis patients may facilitate the pathogenetic process by promoting invasive cell growth via Rac1, MMP3, and ATF-2. PMID: 27041028 Sdc4 has been identified as a mycobacterial attachment receptor on alveolar epithelial cells. PMID: 27279134 Study has demonstrated that SDC-4 expression was increased in sera and skin of atopic dermatitis (AD) patients, suggesting that SDC-4 may contribute to the development of AD. PMID: 27591995 the present study demonstrated that synd4 was involved in the chemotactic migration of ECs in vitro and in vivo. PMID: 27541034 results suggest that SDC4 alleles affect lipid profile in elderly subjects and may in part mediate the link between LDL-C and longevity. PMID: 26254886 Synd4 shedding is a molecular pathological alteration in the development and maintenance of inflammation-associated atrial fibrillation. PMID: 26261514 Dynamic catch of a Thy-1-integrin alpha5beta1+syndecan-4 trimolecular complex explains extraordinary cancer cell adhesion to the vascular endothelium. PMID: 25216363 Syndecan-4 polymorphisms were associated with essential hypertension, body mass index, and coronary artery disease prevalence in the Tampere adult population cardiovascular risk study. PMID: 25410619 No association was found with SDC4 and breast cancer. PMID: 25361632 Data indicate that depletion of syndecan 4 (S4) in umbilical vein endothelial cells (HUVECs) using shRNA inhibited flow-induced alignment in vitro, which was rescued by re-expression of S4. PMID: 25404299 define a role for TG2 activity at the surface of human macrophages in multiple stages of AC clearance and we propose that TG2, in association with heparan sulphates may exert its effect on AC clearance via a mechanism involving the crosslinking of CD44 PMID: 25449226 syndecan 4 is the predominant heparan sulfate proteoglycan in the glomerular endothelial cells glycocalyx PMID: 25122554 TGM2 up-regulation along with ITGB1 and SDC4 plays an important role in the development of RCC tumors and advanced RCC with metastasis PMID: 23499501 Sdc4 is necessary for activation of the integrin during chemokinesis induced by EGF. PMID: 25202019 in healthy discs, SDC4, through its heparan sulfate side chains, contributes to maintenance of the hypoxic tissue niche by controlling baseline expression of Sox9. PMID: 24558194 High glucose modifies TRPC6 channels and ROS production via SDC-4 in human podocytes. PMID: 24942878 The cytoplasmic V domain of syndecan-4 made a significant contribution to the cellular uptake of octaarginine, whereas the cytoplasmic C1 and C2 domains were not involved in the process. PMID: 24632200 syndecan-4 is differentially expressed in seminomas and nonseminomatous germ cell tumours and might be a useful marker. PMID: 23844358 The more upgraded the NYHA grading, the higher level of serum syndecan-4 protein. PMID: 23948417 Syndecan-4 expression and shedding were increased in failing human myocardium. PMID: 23374111 EGFR are the key mediators of SDC-4 expression in MCF-7 cells. PMID: 23374155 Increased expression of SDC4 and FN may be underlying molecular alteration of osteo-sarcoma which accounts for more aggressive clinical behaviour. PMID: 22531343 studies identify S4 as a regulator of MAPK signaling and address the question of how distinct classes of FGFRs individually contribute to signal transduction in endothelial cells PMID: 22569333 By accruing more syndecan-4 on their surface and also shedding it during Th1 cytokine-driven inflammation, asthmatic airway smooth muscle cells may regulate inflammation and remodeling locally and alter the responsiveness of the airways in asthma. PMID: 22268118 in nucleus pulposus, TNF-alpha and IL-1beta regulate SDC4 expression, which plays a key role in pathogenesis of degenerative disc disease by promoting aggrecan degradation by ADAMTS-5. PMID: 21949132 SD-4 bearing distinct heparan sulfate moieties plays a pathogenic role in Sezary syndrome and may be targeted for treatment. PMID: 21252093 Expression of syndecan 4 in healthy human breast tissue during menstrual cycle. PMID: 20398359 In dendritic cells, lysophosphatidylcholine-induced phosphorylation of SDC4 by PKCdelta results in a functional inactivation of SDC4 and decreases adhesion and motility. PMID: 20607801 Data found that Wnt5a reduces cell surface levels and promotes ubiquitination and degradation of SDC4. PMID: 20639201 SDC4 promotes cytokinesis in a phosphorylation-dependent manner in MCF-7 breast adenocarcinoma cells. The serine179-phosphorylation and the ectodomain shedding of SDC4 changed periodically in a cell cycle-dependent way. PMID: 20229236 The expression of syndecan-4 protein was significantly enhanced by TNF-alpha in HUVECs. PMID: 17545042 The down-regulation of syndecan-4, a heparan sulfate proteoglycan, decreased SDF-1/CXCL12-mediated HeLa cell invasion. PMID: 19695308 Study identified an overrepresentation of focal amplifications of known (FGFR3, CCND1, MYC, MDM2) and novel candidate genes (MYBL2, YWHAB and SDC4) in stage Ta bladder carcinoma. PMID: 19821490 Syndecan-4 may be a sensor of tension exerted on the matrix [review] PMID: 19538537 Syndecan-4 mediates antithrombin-induced chemotaxis of human peripheral blood lymphocytes and monocytes PMID: 11801740 Clustering induces redistribution of syndecan-4 core protein into raft membrane domains PMID: 11889131 Syndecan-4 core protein mediates the effects of fibroblast growth factor (FGF)2 on cell function. PKCalpha activation and PDZ-mediated formation of a serine/threonine phosphatase-containing complex by syndecan-4 are downstream events of FGF2 signaling. PMID: 12011116 SDC4 regulates inositol phospholipid binding and signaling PMID: 12377772 the focal adhesion component alpha-actinin interacts with syndecan-4 in a beta-integrin-independent manner PMID: 12493766 Syndecan-4 can promote cell spreading in a beta(1) integrin-dependent fashion through PKCalpha and RhoA PMID: 12509413 endotoxin-induced adhesion of leukocytes to endothelium can be reversed by ligation of syndecan-4 with antithrombin's heparin-binding site PMID: 14652650 syndecan-4/CXCR4 complex is likely a functional unit involved in SDF-1 binding PMID: 15033938 human trabecular meshwork cells express only syndecan-1, and not syndecan-4, at the cell surface, and focal adhesion and stress fiber formation occur independent of syndecan-4 PMID: 15572366

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.