Recombinant Human SULT2B1 Protein (C-6His)

Name: Recombinant Human SULT2B1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1497NP
Availability: InStock

BL-1497NP: Greater than 85% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Cytosolic Sulfotransferase Family 2B Member 1 is produced by our E.coli expression system and the target gene encoding Met1-Glu311 is expressed with a 6His tag at the C-terminus.
Accession	O00204
Synonym	Sulfotransferase Family Cytosolic 2B Member 1; ST2B1; Sulfotransferase 2B1; Alcohol Sulfotransferase; Hydroxysteroid Sulfotransferase 2; SULT2B1; HSST2
Gene Background	Sulfotransferase Family Cytosolic 2B Member 1(SULT2B1) is a member of the Sulfotransferase 1 family. SULT2B1 is highly expressed in the placenta, prostate, and trachea with lower expression levels in the small intestine and lung. SULT2B1 catalyzes the sulfate conjugation of numerous hormones, neurotransmitters, drugs, and xenobiotic compounds. Sulfonation enhances the water solubility of molecules, and therefore their renal excretion, however SULT2B1 can also result in bioactivation to form active metabolites.
Molecular Mass	36.6 KDa
Apmol Mass	35 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM Nacl, 1mM EDTA, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 85% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Responsible for the sulfation of cholesterol. Catalyzes sulfation of the 3beta-hydroxyl groups of steroids, such as, pregnenolone and dehydroepiandrosterone (DHEA). Preferentially sulfonates cholesterol, while it has also significant activity with pregnenolone and DHEA. Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation.; Sulfonates pregnenolone but not cholesterol.
Subcellular Location	Cytoplasm, cytosol. Microsome. Nucleus.
Protein Families	Sulfotransferase 1 family
Database References	HGNC: 11459 OMIM: 604125 KEGG: hsa:6820 STRING: 9606.ENSP00000201586 UniGene: PMID: 29701841 Mutation in SULT2B1 leads to an autosomal-recessive congenital ichthyosis phenotype via increased proliferation of human keratinocytes, thickening of epithelial layers, and altered epidermal cholesterol metabolism. PMID: 28575648 Since 1'-hydroxyestragole exposure readily produced DNA injury in B-13/H cells, these data suggest that cholangiocarcinomas generated in rats fed estragole may be dependent, in part, on SULT2B1 activation of the 1'-hydroxyestragole metabolite. PMID: 26739637 Overexpression of SULT2B1b is an independent prognostic indicator and promotes cell growth and invasion in colorectal carcinoma. PMID: 26121319 This study identified specific germline variations in estradiol metabolism-related pathways, namely CYP1B1, SULT2B1, and HSD17B2, as novel prognostic markers that are cumulatively associated with increased risk of prostate cancer progression PMID: 24682418 The SULT2B1 isoforms have a unique 50 amino acid carboxy-terminal sequence that is not present in the other human SULT isoforms (review). PMID: 24020383 Data indicate that cholesterol sulfate (CS) and SULT2B1b inhibited gluconeogenesis by targeting the gluconeogenic factor hepatocyte nuclear factor 4alpha (HNF4alpha) in both cell cultures and human SULT2B1b transgenic mice. PMID: 24277929 SULT2B1 is induced by calcitriol activated vitamin D receptor in human prostate cancer cells. PMID: 23579488 SULT2B1b expression promotes proliferation of hepatocellular carcinoma cells in vitro and in vivo, which may contribute to the progression of HCC. PMID: 23593328 In HT29 cells expression of SULT2B1 was enhanced by probiotic fermentation supernatants without aleurone. PMID: 21840698 The Ca(2+)-bound form may be physiologically relevant for stressed cells with an elevated free calcium level. PMID: 20329768 The expression of SULT2B1b mRNA in endometrial stromal cells is induced by progesterone and that in endometrial epithelial cells is induced by relaxin via the cAMP pathway. PMID: 19243756 alternative splicing of SULT2B1 results in cholesterol sulfotransferase or pregnenolone sulfotransferase PMID: 12145317 crystal structure of hydroxysteroid sulfotransferases SULT2B1a and SULT2B1b bound to the substrate donor product 3'-phosphoadenosine 5'-phosphate and acceptor substrate pregnenolone revealed a different catalytic binding orientation PMID: 12923182 We have documented the presence of the enzyme (SULT2B1b) that sulfonates cholesterol in human platelets and examined the influence of plasma lipoproteins on the expression and activity of this enzyme PMID: 14676143 Differential subcellular localization of SULT2B1b in prostate and placenta suggests that SULT2B1b may be differentially regulated and have different physiological functions in these two hormonally responsive human tissues PMID: 14741047 confinement of SULT2B1b to the granular layer coincides with this being the area with the highest cholesterol sulfate content suggesting that the physiologic action of cholesterol sulfate is likely carried out in this region of the living epidermis PMID: 15140224 Immunohistochemical localization of SULT2B1b in human tissues showed expression in the cytoplasm of ciliated columnar or cuboidal epithelial cells in terminal bronchia. PMID: 15878639 Of five Sp1 elements identified in SULT2B1, two had regulatory activity utilizing immortalized human keratinocytes. PMID: 15953604 SULT2B1b, by acting on a variety of oxysterols, offers a potential pathway for modulating in vivo the injurious effects of these compounds. PMID: 17347498 Results show SULT2B1 polymorpohisms affect enzyme functions. PMID: 17496163 SULT2B1 is involved in reulating prostate cell responsiveness to dehydroepiandrosterone and delta5-androstenediol. PMID: 17626250 Data show that SULT2B1b is localized in cytosol and nuclei of cancerous and normal breast tissue, and that African Americans had significantly more SULT2B1b in nuclei of epithelial cells of normal breast tissue compared to Caucasians. PMID: 18586095

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Human SULT2B1 Protein (C-6His)

Recombinant Human SULT2B1 Protein (C-6His)

Product Overview

Target Details

FAQs