Recombinant Human Substance-P Receptor (TACR1) Protein (GST)

Name: Recombinant Human Substance-P Receptor (TACR1) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-05194P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Substance-P Receptor (TACR1) Protein (GST) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P25103
Target Symbol	TACR1
Synonyms	Neurokinin 1; Neurokinin 1 Receptor; NK 1 receptor; NK 1R; NK-1 receptor; NK-1R; NK1 receptor; NK1R; NK1R_HUMAN; NKIR; SPR; Substance P receptor; Substance-P receptor; TAC 1R; TAC1R; Tachykinin 1 receptor (substance P receptor neurokinin 1 receptor); Tachykinin receptor 1 (substance P receptor neurokinin 1 receptor); Tachykinin receptor 1; TACR1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	MDNVLPVDSDLSPNISTNTSEPNQFVQPA
Expression Range	1-29aa
Protein Length	partial
Mol. Weight	30.1kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family
Database References	HGNC: 11526 OMIM: 162323 KEGG: hsa:6869 STRING: 9606.ENSP00000303522 UniGene: PMID: 30301182 NK-1RA is recommended to use in carboplatin-based chemotherapy, not oxaliplatin-based chemotherapy PMID: 30019968 Study revealed extensive distribution of SP and NK1R in nuclei intimately related to cardiorespiratory function and autonomic control, with significant co-distribution and co-localization with 5-HT in the medullary network in the normal human infant during development. A trend for NK1R binding to decrease with age was observed with significantly higher binding in premature and male infants. PMID: 29331576 These results reveal a critical role for endosomal signaling of the NK1R in the complex pathophysiology of pain. PMID: 28566424 The percentages of SP+ and NK1R+ expression populations of monocytes, helper T cells, natural killer T cells and basophils in peripheral blood of eczema patients were markedly elevated. PMID: 28154998 Expression of NK1R increases in the eosinophils of chronic spontaneous urticaria (CSU) patients. Blockers of NK1R might be used for CSU treatment. PMID: 28871952 NK-1R may positively regulate melanogenesis through Wnt/beta-catenin signaling pathway. PMID: 27835606 This study revealed for the first time an increase of Mast Cells -nerve association and NK1R expression on Mast Cells during Allergic Rhinitis as well as nerve fibres containing receptors for mass cells. PMID: 28030866 Kinase activation led to increased MMP-2 and MT1-MMP expression and melanoma cell migration induced by hHK-1. Thus, hHK-1 and the NK1 receptor are critical to melanoma cell migration and each may be a promising chemotherapeutic target PMID: 27458061 Within a small cohort of patients who presented for treatment,a novel gene was identified that appears to contribute to anorexia nervosa pathophysiology, TACR1. PMID: 28291261 NK1 receptor expression is increased in colonic tissues from experimental colitis models and in the colon of Irritable Bowel Disease patients. PMID: 28774868 High Truncated Neurokinin-1 Receptor expression is associated with Neuroblastoma. PMID: 29061788 Abnormalities in substance P neurokinin-1 receptor binding in key brainstem nuclei is associated with sudden infant death syndrome. PMID: 28931039 TGF-beta1 down-regulates the gene expression of the full-length variant of NK-1 R in human keratocytes, which might impact its signaling pathway and thus explain the known delay in internalization after activation by SP seen with TGF-beta1 treatment. PMID: 26673553 results suggest that ARRB1 plays an essential role in NK1R-mediated cell proliferation and G2/M transition in glioblastoma cells. Interference with ARRB1-mediated signaling via NK1R may have potential significance for therapeutic strategies targeting glioblastoma. PMID: 28341744 up-regulated in nasal mucosa at contact point in intranasal mucosal contact point headache PMID: 26817501 We showed that pretreating a cardiomyocyte cell line (H9C2) and a TNBC cell line (MDA-MB 231) with aprepitant, a SP receptor antagonist that is routinely used to treat chemotherapy-associated nausea, decreased DOX-induced reduction of viability, apoptotic cell death, and ROS production in cardiomyocytes PMID: 26981525 These findings reveal important insight into the molecular mechanisms of the NK1R complex as a critical component in a model of pediatric liver cancer and may support the development of novel therapeutic interventions for hepatoblastoma PMID: 26516161 Report absence of full-length neurokinin-1 receptor protein expression by cutaneous T cells in mycosis fungoides. PMID: 25783846 Inhibition of CaV2.3 channels by NK1 receptors is sensitive to membrane cholesterol but insensitive to caveolin-1. PMID: 25204428 NK-1 activation could enhance EGFR expression and phosphorylation, and then activate EGFR related Erk/MAPK and Akt pathway. PMID: 25817575 The single nucleotide polymorphisms in the TACR1 gene are associated with sex differences in postoperative nausea and vomiting. PMID: 26012530 Results show the existence of a relevant alteration in NK-1R expression in bipolar disorder patients and further suggest Substance P involvement in BD. PMID: 25841180 Immunohistochemical NK-1R and pEGFR positivity with VDR negativity can be used to identify areas of sporadic colorectal neoplasia. PMID: 25684939 Hepatoblastoma cells predominantly expressed the truncated splice variant of NK1R. PMID: 24412605 Findings indicate a role of the TACR1 gene in the development of sicca syndrome in subjects affected with fibromyalgia. PMID: 25786041 NK-1R activation by substance P stimulation promotes growth and migration of esophageal squamous cell carcinoma cells. PMID: 25659767 Different NK1R subtype profiles of glomerular basement membrane (GBM) cell lines appear to influence the binding of SP conjugates and their cell internalization properties. PMID: 24552486 Down-regulation of NK1R-Tr may be a key factor in maintaining the quiescent phenotype of breast cancer cells among bone marrow stroma. PMID: 25130457 The association with TACR1 and bipolar affective disorder, alcohol dependence syndrome, and attention-deficit hyperactivity disorder suggests a shared molecular pathophysiology between these affective disorders. PMID: 24817687 NK-1 receptors are expressed in uveal melanomas and uveal melanoma cell lines. PMID: 24548567 TACR1 expression level was differentially altered in Crohn disease and ulcerative colitis patients. PMID: 23827863 Residues 11-21 and residues 170-193 of NK1 receptor are binding sites for substance P. PMID: 24831006 the primary function of betaARRs and ECE-1 in SP-dependent inflammatory signaling is to promote resensitization, which allows the sustained NK1R signaling from the plasma membrane that drives inflammation PMID: 24898255 we demonstrated for the first time that the NK1R is expressed in human osteosarcoma cell line MG63 and that this receptor can be targeted with NK1R antagonists both in vitro as well as in vivo. PMID: 24190675 data confirm the overall inflammatory nature of HIV-associated neuropathology, and a decrease in the expression of SP and NK1R-F. PMID: 23916293 NK1 receptors may be activated, or up-regulated in the peripheral, painful tissue of a chronic pain condition. PMID: 24155873 It seems that a common mechanism for cancer cell proliferation mediated by SP and the NK-1 receptor is triggered, as well as a common mechanism exerted by NK-1 receptor antagonists on tumor cells, i.e. apoptosis. PMID: 23933301 Roles of full-length and truncated neurokinin-1 receptor on tumor progression and distant metastasis in human breast cancer. PMID: 23807418 This suggests that potential pharmacological interventions that target NK-1R signaling may be most effective for treating distinct symptoms in IBD and IBS. PMID: 23582152 High NK-1 Receptor expression is associated with breast cancer. PMID: 24030979 NK1R forms physical complexes with NET. PMID: 23979140 Each of the 5 single nucleotide polymorphism(SNP)s in the TACR1 gene is significantly related to alcohol dependence severity. PMID: 23078527 Our results suggest that SP plays an important role in the development of pancreatic cancer metastasis and PNI, and blocking the SP/NK-1R signaling system is a novel strategy for the treatment of pancreatic cancer. PMID: 23345604 NK1R expression is induced in ectopic endometrial tissue by peritoneal TNFalpha. PMID: 23553861 NK1R expression correlates positively with plasma insulin, fatty acid, and complement C3 and negatively with adiponectin, CEBPalpha, CEBPbeta, and peroxisome proliferator-activated receptor-gamma mRNA expression in omental, but not subcutaneous, adipose tissue. PMID: 23257919 PAR(2) has a higher apparent affinity for both beta-arrestins than does NK1R, recruits them at a faster rate, and exhibits more rapid desensitization of the G-protein signal. PMID: 23235155 NK1R is a potential regulator of human glioma cell migration by the up-regulation of MMP-2 and MT1-MMP PMID: 23166329 Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells PMID: 23024816 investigation of age and sex influences on NK1 receptor expression in brain tissues PMID: 22225860

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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