Recombinant Human SPP1 Protein (C-6His)

Name: Recombinant Human SPP1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0536NP
Availability: InStock

BL-0536NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality cytokines for advanced research, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Secreted Phosphoprotein 1 is produced by our Mammalian expression system and the target gene encoding Ile17-Asn314 is expressed with a 6His tag at the C-terminus.
Accession	P10451
Synonym	Osteopontin; Bone Sialoprotein 1; Nephropontin; Secreted Phosphoprotein 1; SPP-1; Urinary Stone Protein; Uropontin; SPP1; BNSP; OPN
Gene Background	Secreted Phosphoprotein 1 (SPP1) is a secreted multifunctional glycoprotein. Its putative functions include roles in bone metabolism, immune regulation, wound healing, cell survival, and tumor progression. Based on gene structure and chromosomal location, SPP1 is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP), enamelin (ENAM), and matrix extracellular phosphoglycoprotein (MEPE). SPP1 is expressed in bone, although it is also expressed in other tissues. SPP1 acts as a cytokine that is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteopontin has been implicated as an important factor in bone remodeling. Specifically, research suggests it plays a role in anchoring osteoclasts to the mineral matrix of bones. The fact that SPP1 interacts with multiple cell surface receptors which are ubiquitously expressed makes it an active player in many physiological and pathological processes including wound healing, bone turnover, tumorigenesis, inflammation and ischemia. Therefore, manipulation of plasma Osteopontin levels may be useful in the treatment of autoimmune diseases, cancer metastasis, osteoporosis and some forms of stress.
Molecular Mass	34.75 KDa
Apmol Mass	60-70 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Subcellular Location	Secreted.
Protein Families	Osteopontin family
Database References	HGNC: 11255 OMIM: 166490 KEGG: hsa:6696 STRING: 9606.ENSP00000378517 UniGene: PMID: 29463954 Study demonstrated that osteopontin exerts an important role in the monocytes/macrophage phenotypic differentiation from hypertensive patients with vascular calcification. PMID: 28091516 The expression of OPN was high in endometrium in secretory phase and in vitro decidualized endometrial stromal cells. Further analysis confirmed that OPN expression was upregulated by cAMP and C/EBPbeta signal pathway, while downregulated by miR181b. Increased OPN expression could promote the expression of decidualization-related and angiogenesis-related genes PMID: 29420252 Results of the study showed that low urinary OPN levels were correlated with increased kidney stone risk, and dietary habits can affect urinary OPN level PMID: 30114399 The serum levels of cystatin C and urine NGAL, urine OPN can be used as a good marker for assessing the renal effect of obesity which can lead end stage renal disease in pediatric population. PMID: 30035656 novel 9175th- (exon 7) position polymorphism of OPN and rs17524488 were related to susceptibility to ankylosing spondylitis in a Chinese population PMID: 29581970 Over-expressed and hypo-methylated SPP1 gene is associated with hepatocellular carcinoma. PMID: 27760737 Serum osteopontin concentrations were increased in acute pancreatitis (AP) compared with follow-up 3 months after discharge. PMID: 29424811 Our data provide evidence that leptin-mediated OPN upregulation promote TH2 inflammation in AR and this process is achieved through the alpha4 integrin and PI3K/AKT signaling pathways. PMID: 29885866 miR-129-5p level was decreased in fibrotic liver of human, and reduced by rOPN treatment. In contrast, miR-129-5p was induced in HSCs transfected by OPN siRNA. These data suggested that OPN induces Col 1 expression via suppression of miR-129-5p in hepatic stellate cells. PMID: 29196165 High circulating osteopontin levels predict major adverse cardiovascular events in patients with severe carotid artery stenosis. PMID: 29317141 High SPP1 Expression is associated with Hepatocellular Carcinomas. PMID: 29693976 Serum OPN and DKK1 levels of hepatocellular carcinoma patients could be considered as novel biomarkers showing prognostic significance after hepatectomy based on long-term survival data PMID: 29753515 Upregulation of ADIPOR1 and SPP1, among the adipokine gene family, in cancer tissue is associated with poor survival in CRC, suggesting a potential mechanism linking obesity and colorectal cancer. PMID: 29761507 Review/Meta-analysis: report a significantly higher circulating OPN level in systemic lupus erythematosus patients, a trend of positive correlation between OPN levels and SLE activity, and a significant association between OPN 1239 C/A and 9250 C/T polymorphisms, and SLE development. PMID: 27307447 OPN is not only the pivotal protein controlling fibrosis, but may also serve as a biomarker associated with prognosis. PMID: 29120858 Our data show that the ability of AML cells to constitutively release high levels of osteopontin is associated with a favorable prognosis, and this is different from previous studies of osteopontin mRNA levels. PMID: 27739925 First report of OPN cleavage in THP-1 macrophages after PMA (phorbol 12-myristate 13-acetate) stimulation, and of enhanced cleavage induced by BCG infection (bovine tuberculosis). PMID: 29385060 The results suggest that OPN, MMP9 and S100A8 all play a significant role in bladder cancer progression and are potential prognostic markers and therapeutic targets in bladder cancer PMID: 29209142 We demonstrated an important effect of the SPP1 rs4754 on subclinical markers of carotid atherosclerosis in subjects with T2DM; however, as demonstrated by the multiple linear regression analysis, neither rs4754 nor rs28357094 had an important impact on the progression of subclinical markers of carotid atherosclerosis in subjects with T2DM. PMID: 28990744 these results showed that expression of tear OPN protein by perennial allergic conjunctivitis patients increased significantly compared with controls or seasonal allergic conjunctivitis patients out of the pollen season PMID: 29279263 The study revealed an enhanced sensitivity of aortic valve interstitial cells to osteogenic inductors in aortic stenosis patients, which indicates probable implication of OPN, OPG, and BMP2 genes in pathogenesis of aortic valve calcification. PMID: 29308559 Carriers of the risky genotype or haplotype also had increased gastric OPN expression (p = 0.038) and inflammation (p = 0.007). SPP1 polymorphisms predispose to intestinal metaplasia development in H. pylori infected males. PMID: 28685609 Respiratory syncytial virus infection leads to increased OPN expression.IL-1beta is involved in the regulation of OPN levels during respiratory syncytial virus infection. PMID: 29677209 plasma level increases in pregnancy independently of asthma PMID: 29200898 Methylglyoxal-bis-guanylhydrazone may have potential therapeutic utility in reducing or normalizing OPN levels and regulating monocyte activation in diseases that involve chronic inflammation. PMID: 29538412 These results might be considered a useful starting point to validate OPN as a prognostic and diagnostic marker of hepatocellular carcinoma. PMID: 28711012 study demonstrates that the OPN gene SNP rs1126616 is statistically associated with cerebral palsy. PMID: 27114095 LAMP3 promotes the invasion of osteosarcoma cells via SPP1 signaling. PMID: 28849219 Extended liver surgery is the only potentially curative treatment for patients with cholangiocarcinoma (CCA/biliary cancer), but it is currently unclear which patients benefit most from surgery. Detecting serum levels of osteopontin - a specific secreted glycoprotein involved in multiple diseases - in CCA patients might help to identify those patients that particularly benefit from tumour resection. PMID: 28668580 MALAT1 could directly bind to miR-127-5p to inhibit its expression, so as to rescue OPN expression and promote chondrocyte proliferation through PI3K/Akt pathway. PMID: 28590075 A linear negative correlation between serum OPN and total bone mineral density (BMD) of Type I diabetes mellitus patients compared to control group was noticed, indicating that the amount of serum OPN could be effective on BMD and a good predicting factor for osteoporosis PMID: 28499311 OPN regulates CYP7A1 levels and the metabolic fate of liver acetyl-CoA as a result of CHOL and PC metabolism interplay PMID: 28754826 these findings demonstrated that OPN and VEGF were overproduced in nasal polyps and that OPN induced VEGF production, which indicated that OPN-VEGF axis might contribute to angiogenesis in nasal polyps PMID: 28716167 Osteopontin and CD44 play important roles in the development and progression of meningioma and can be used as prognostic markers for tumor recurrence and progression as well as therapeutic targets for the development of new drugs. PMID: 29504367 OPN play a role in pathogenesis of neurodegenerative diseases or in neuroprotection by regulating the activation and function of microglia. PMID: 28698867 osteopontin has a role in DNA repair and impact on human glioblastoma radiosensitivity PMID: 27563812 SPP1 promoted the metastasis of CRC. PMID: 28531945 Results show that osteopontin is positively associated with hepatocellular carcinoma (HCC) metastasis, and that OPN can activate CCR1 expression through the PI3K/AKT/HIF-1a signaling pathway to promote HCC progression and metastasis. PMID: 29285854 This review presents the current knowledge on the expression profiles of OPN and its main splice variants in human cancers, as well as the potential implications in patient outcome. [review] PMID: 28440483 Strong correlations between the expression of type I, II, IV collagen and osteopontin and the clinical stage of tympanosclerosis indicate the involvement of these proteins in excessive fibrosis and pathological remodeling of the tympanic membrane. PMID: 29068597 result of this study confirms that increased levels of OPN exist in CSF and peripheral blood of MS patients and strengthens the evidence regarding the clinical utility of OPN as a promising and validated biomarker for MS PMID: 29346446 OPN overexpression was correlated with poor overall survival and clinical features reflecting high aggressiveness in patients with gastric cancer. PMID: 27626167 ITGbeta3 and CD44 expression levels determine whether OPN-a inhibits or enhances growth in lung cancer cells. PMID: 27487131 Study identified that miR-127-5p targets the 3' UTR of osteoarthritis (OA) mRNA to down-regulate the expression of OPN. In OA, the down-expressed miR-127-5p allows the expression of OPN, which mediates the establishment and development of OA. PMID: 27126955 Our study shows a strong effect of the rs28357094 G allele in increasing osteopontin expression in the presence of deflazacort, and adds to the evidence that rs28357094 polymorphism may predict response to glucocorticoids in Duchenne muscular dystrophy. PMID: 28595270 we described the expression pattern of osteopontin splice variants in papillary thyroid carcinoma samples and the key role of osteopontin-a expression on activating tumor progression features PMID: 27409830 In Osteoarthritis tissues, OPN mRNA, and NEAT1 expression was upregulated, whereas miR-181c expression was downregulated, indicating that targeting NEAT1 to rescue miR-181c expression so as to inhibit OPN expression and synoviocyte proliferation PMID: 28379604 IL-6 and sIL-6R induced by IL-1beta may trigger IL-6 trans-signaling, contributing to the upregulation of OPN in THP-1 macrophages. Macrophages may be used as a source of IL-6 and sIL-6R and evoke IL-6 trans-signaling. PMID: 27863335 OPN is a key mediator of intracerebral tumor growth, invasion, and dissemination in central nervous system lymphoma, and these effects depend upon activation of NF-kappaB. PMID: 27050077

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human SPP1 Protein (C-6His)

Recombinant Human SPP1 Protein (C-6His)

Product Overview

Target Details

FAQs