Recombinant Human Sialidase-3 (NEU3) Protein (His)

Name: Recombinant Human Sialidase-3 (NEU3) Protein (His)
Brand: Beta LifeScience
SKU: BLC-05078P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Sialidase-3 (NEU3) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9UQ49
Target Symbol	NEU3
Synonyms	NEU3Sialidase-3; EC 3.2.1.18; Ganglioside sialidase; Membrane sialidase; N-acetyl-alpha-neuraminidase 3
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	EEVTTCSFNSPLFRQEDDRGITYRIPALLYIPPTHTFLAFAEKRSTRRDEDALHLVLRRGLRIGQLVQWGPLKPLMEATLPGHRTMNPCPVWEQKSGCVFLFFICVRGHVTERQQIVSGRNAARLCFIYSQDAGCSWSEVRDLTEEVIGSELKHWATFAVGPGHGIQLQSGRLVIPAYTYYIPSWFFCFQLPCKTRPHSLMIYSDDLGVTWHHGRLIRPMVTVECEVAEVTGRAGHPVLYCSARTPNRCRAEALSTDHGEGFQRLALSRQLCEPPHGCQGSVVSFRPLEIPHRCQDSSSKDAPTIQQSSPGSSLRLEEEAGTPSESWLLYSHPTSRKQRVDLGIYLNQTPLEAACWSRPWILHCGPCGYSDLAALEEEGLFGCLFECGTKQECEQIAFRLFTHREILSHLQGDCTSPGRNPSQFKSN
Expression Range	2-428aa
Protein Length	Partial
Mol. Weight	52.1kDa
Research Area	Metabolism
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes.
Subcellular Location	Cell membrane; Peripheral membrane protein. Membrane, caveola. Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein.
Protein Families	Glycosyl hydrolase 33 family
Database References	HGNC: 7760 OMIM: 604617 KEGG: hsa:10825 STRING: 9606.ENSP00000294064 UniGene: PMID: 28760640 effect of overexpression on EGFR downstream pathway activation and TKI targeted therapies sensitivity in non-small cell lung cancer cell lines PMID: 29088281 The most potent compound tested targeted the human Neu4 isoenzyme, and was able to substantially reduce the rate of cell migration. We found that the lateral mobility of integrins was reduced by treatment of cells with Neu3, suggesting that Neu3 enzyme activity resulted in changes to integrin-co-receptor or integrin-cytoskeleton interactions PMID: 27344026 NEU3 possesses the ability to interact with specific proteins, many of which are different in each cell compartment, supporting NEU3 involvement in the cell stress response, protein folding, and intracellular trafficking. PMID: 26987901 findings identify NEU3 as a participant in head and neck squamous cell carcinoma progression PMID: 26470851 Besides the already reported indirect EGFR activation through GM3, sialidase NEU3 could also play a role on EGFR activation through its desialylation. PMID: 25922362 NEU3 promotes anchorage-independent growth and in vivo tumorigenicity. PMID: 25803810 In the plasma membrane, NEU3 has a role in endocytosis and protein binding at the cell surface. PMID: 26251452 Results indicate an essential contribution of NEU3 to tumorigenic potential through maintenance of stem-like characteristics of colon cancer cells by regulating Wnt signaling at the receptor level. PMID: 25810027 The results provide strong evidence that the serum sialidase is, in fact, NEU3, and this subtype may, therefore, be a potential utility for novel diagnosis of human cancers PMID: 25652216 Studies indicate that ganglioside-specific sialidase NEU3 cleaves sialic acids preferentially from gangliosides. PMID: 25408341 Weak anchorage of NEU1 and NEU3 to the plasma membrane and their loss during erythrocyte life could be a tool to preserve the cellular sialic acid content to avoid early aging of erythrocyte and processes of cell aggregation in the capillaries. PMID: 22903576 NEU3 is a key regulator of the beta1 integrin-recycling pathway and FAK/AKT signaling and demonstrate its crucial role in renal cell carcinomas malignancy. PMID: 23139422 Data indicate that sialidases Neu1 and Neu3 are present on sperm, and their activity is required for capacitation and zona pellucida binding. PMID: 22989879 Data show that NEU1 was immunolocalized to both the plasma membrane and the perinuclear region, and NEU3 was detected both in the cytosol and nucleus. PMID: 22403397 These data suggest that NEU3 regulates tumor progression through AR signaling, and thus be a potential tool for diagnosis and therapy of androgen-independent prostate cancer. PMID: 21681193 NEU3 is highly expressed to enhance malignant phenotypes including apoptosis inhibition in malignant melanomas. PMID: 21895867 observed that trisaccharides with incorporated azide groups in the Neu5Ac residue at either C9 or the N5-Ac position were substrates, and in the case of the N5-azidoacetyl derivative, the activity was superior to that of GM3 PMID: 21675735 Truncations at the N- or C-terminus of more than 10 residues abolished enzyme activity. PMID: 20511247 NEU3 expression is diversely regulated by Sp1/Sp3 transcription factors binding to alternative promoters, which might account for multiple modulation of gene expression. PMID: 20518744 Data show that Neu3 could represent a new potent biomarker in childhood ALL, to assess the efficacy of therapeutic protocols and to rapidly identify an eventual relapse. PMID: 19588508 Neu3 functions as a caveolin-related signaling molecule within caveolin-rich microdomains PMID: 12011038 up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression PMID: 12149448 NEU3 participates in the control of insulin signaling via modulation of gangliosides and interaction with Grb2 PMID: 12730204 Results show that the sialidase activity of human membrane type ganglioside sialidase (Neu3)is specific for gangliosides. PMID: 15179041 Data show that the differentiation of monocytes into macrophages is associated with regulation of the expression of at least three distinct cellular sialidases, Neu1, 3, and 4, with specific up-regulation of the enzyme activity of only Neu1. PMID: 15885103 NEU3 differentially regulates cell proliferation through integrin-mediated signalling depending on the extracellular matrix and, on laminins, NEU3 activated molecules often up-regulated in carcinogenesis. PMID: 16241905 NEU3 activated by IL-6 exerts IL-6-mediated signaling, largely via the PI3K/Akt cascade, in a positive feedback manner and contributes to expression of a malignant phenotype in renal cell carcinoma PMID: 16428383 NEU3 is able to mobilize to membrane ruffles in response to growth stimuli and activate the Rac-1 signaling by co-localization with Rac-1, leading to increased cell motility. PMID: 16765317 The activity of Neu3 changed minimally in activated lymphocytes. PMID: 17028199 NEU3 is an important regulator of insulin sensitivity and glucose tolerance. PMID: 17292733 NEU3 may be an essential gene for cancer cell survival. PMID: 17334392 Neu3 is up-regulated in Jurkat cells undergoing etoposide-induced apoptosis PMID: 17827720 NEU3 is markedly up-regulated in many types of cancers including colon and renal carcinomas and suppresses apoptosis of cancer cells. PMID: 18023981 Neu3 degrades membrane sialic acids resulting in downregulation of the PKC/ERKs/p38 MAPK signaling pathway, causing a decrease in CD41b surface antigen expression and inhibition of megakaryocytic differentiation of K562 cells. PMID: 18339327 related to malignancy and may be potential targets for cancer diagnosis and therapy PMID: 18651674 upon Neu3 silencing, K562 cells show a decrease in proliferation, propensity to undergo apoptosis, and megakaryocytic differentiation. PMID: 18820643 increased CD15 expression is not due to de novo biosynthesis of CD15, but results predominantly from induction of alpha(2-3)-sialidase activity PMID: 18953356 occurrence of Neu3 in the inner membrane and Neu1 in the outer membrane of the nuclear envelope PMID: 19686243

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Human Sialidase-3 (NEU3) Protein (His)

Recombinant Human Sialidase-3 (NEU3) Protein (His)

Product Overview

Target Details

FAQs