Recombinant Human Serum Albumin/HSA Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0025UL
Carrier effect of rHSA is observed in the cell proliferation in TF-1 human cells treated with Recombinant Human IL-3 in the presence of 25 µg/mL rHSA. The carrier effect due to rHSA does not alter the ED50 in this assay
Carrier effect of rHSA is observed in the cell proliferation in TF-1 human cells treated with Recombinant Human IL-3 in the presence of 25 µg/mL rHSA. The carrier effect due to rHSA does not alter the ED50 in this assay

Recombinant Human Serum Albumin/HSA Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0025UL
/
Size

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Product Overview

Product Name Recombinant Human Serum Albumin/HSA Protein
Target Symbol ALB
Alternative Names Albumin, HSA
Expression System HEK cell line, with no Fetal Bovine Serum (FBS)
Molecular Weight 66.5 kDa (monomer, predicted) observed on SDS-PAGE gel stained with stained using Coomassie® Blue under reducing conditions
Sequence Asp25-Leu609
Purity >98% under reducing conditions by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity The carrier effect of Recombinant Human Serum Albumin/HSA was determined by enhancement of cell proliferation using TF-1 human cells. Stout, et al. (2022) Commun Biol 5:466. Cell proliferation in TF-1 human cells was induced in a dose-dependent manner by either Recombinant Human GM-CSF or Recombinant Human IL-3 and demonstrated >25% enhancement in the presence of rHSA.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.005 EU/1ug (<1 EU/mg) of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 1 mg/mL in sterile PBS.
Target Function Human serum albumin (HSA) is a ~66.5 kDa, non-glycosylated plasma protein that contributes critically to physicochemical homeostasis in biochemical and cell culture systems through regulation of osmotic pressure and pH buffering capacity. HSA functions as a high-capacity carrier protein with multiple ligand-binding domains, enabling reversible binding and transport of a wide range of hydrophobic and amphipathic molecules, including long-chain fatty acids, steroid and thyroid hormones, bilirubin, and divalent metal ions. In addition to its transport functions, HSA exhibits protein-stabilizing activity by limiting denaturation, aggregation, and proteolytic degradation of labile proteins and enzymes. Owing to these combined buffering, carrier, and protective properties, recombinant HSA effectively recapitulates many of the essential biological functions of serum when used in chemically defined cell culture media.
Tissue Specificity Plasma
Cellular Localization Secreted protein

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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