Recombinant Human Scavenger Receptor Class B Member 1 (SCARB1) Protein (His-SUMO)

Name: Recombinant Human Scavenger Receptor Class B Member 1 (SCARB1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03628P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Scavenger Receptor Class B Member 1 (SCARB1) Protein (His-SUMO) is produced by our E.coli expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q8WTV0
Target Symbol	SCARB1
Synonyms	CD36 and LIMPII analogous 1; CD36; CD36 Antigen like 1; CD36 antigen-like 1; CD36L1; CLA 1; CLA-1; CLA1; Collagen type I receptor; HDLQTL6; MGC138242; SCARB1; Scavebger Receptor Class B Member 1; Scavenger receptor class B member 1; Scavenger Receptor Class B Type 1; SCRB1_HUMAN; SR BI; SR-BI; SRB1; SRBI; Thrombospondin receptor like 1; thrombospondin receptor-like 1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	PSLIKQQVLKNVRIDPSSLSFNMWKEIPIPFYLSVYFFDVMNPSEILKGEKPQVRERGPYVYREFRHKSNITFNNNDTVSFLEYRTFQFQPSKSHGSESDYIVMPNILVLGAAVMMENKPMTLKLIMTLAFTTLGERAFMNRTVGEIMWGYKDPLVNLINKYFPGMFPFKDKFGLFAELNNSDSGLFTVFTGVQNISRIHLVDKWNGLSKVDFWHSDQCNMINGTSGQMWPPFMTPESSLEFYSPEACRSMKLMYKESGVFEGIPTYRFVAPKTLFANGSIYPPNEGFCPCLESGIQNVSTCRFSAPLFLSHPHFLNADPVLAEAVTGLHPNQEAHSLFLDIHPVTGIPMNCSVKLQLSLYMKSVAGIGQTGKIEPVVLPLLWFAESGAMEGETLHTFYTQLVLMPKVMHY
Expression Range	33-443aa
Protein Length	Extracellular Domain
Mol. Weight	62.7kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Receptor for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux. Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity.; (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry. Binding between SCARB1 and the hepatitis C virus glycoprotein E2 is independent of the genotype of the viral isolate.; (Microbial infection) Mediates uptake of M.fortuitum, E.coli and S.aureus.; (Microbial infection) Facilitates the entry of human coronavirus SARS-CoV-2 by acting as an entry cofactor through HDL binding.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Membrane, caveola; Multi-pass membrane protein. Note=Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
Protein Families	CD36 family
Database References	HGNC: 1664 OMIM: 601040 KEGG: hsa:949 STRING: 9606.ENSP00000261693 UniGene: PMID: 29102450 Findings suggested that polymorphism rs5888 had negative association with coronary heart disease, especially in male. [review] PMID: 30103009 We demonstrate that 4F and Cl/NO2-HDL act on scavenger receptor type I (SR-B1) using human aorta endothelial cells (HAEC) and SR-B1 ((-/-)) mouse aortic endothelial cells. PMID: 29277016 Our results demonstrate that SR-BI functions as an oncogene and promotes progression of clear cell renal cell carcinoma (ccRCC). SR-BI may serve as a potential prognostic biomarker and therapeutic target for ccRCC. PMID: 29357836 SCARB1 gene polymorphisms may serve as a potential predictor of treatment responses in chronic hepatitis C patients receiving interferon-based therapy PMID: 27561198 The S112F single amino acid mutation in SR-BI inhibited the infectivity of hepatitis C virus derived from cell culture in a cell culture model by downregulating the expression of the SR-BI protein. PMID: 28811710 The cell surface receptor SR-BI (scavenger receptor class B member 1), is essential for hepatitis C virus (HCV) entry into hepatocytes. Variations in the gene coding this receptor influence infectivity and viral load. We analyzed these variations to gain a better understanding of inter-individual differences over the course of HCV infection. PMID: 28363797 The SCARB1AA genotype decreased cardiovascular risk and carrying GA genotype and G allele increased the risk of CAD. AA genotype carriers had higher levels of big-sized HDL subfraction. PMID: 28882953 These findings provide new insights into the role of SR-B1 in cellular cholesterol homeostasis and suggest molecular links between SR-B1-dependent lipid sensing and cell cholesterol and lipid droplet dynamics PMID: 29196159 Liposomes modified with both apolipoproteins A-I and E were internalized in HepG2 cells in FBS-depleted culture medium at the same levels as unmodified liposomes in FBS-containing culture medium, which indicates that apolipoproteins A-I and E were the major serum components involved in liposomal binding to SR-B1 or LDLR (or both). PMID: 28888368 Here we show that inhibition of SR-B1 reduced cell survival, migration and invasion, and cholesterol content in NB cell lines. Additionally analysis of SR-B1 levels in NB patient biopsies using the R2: Genomics Analysis and Visualization Platform showed that high SR-B1 expression correlated with decreased overall and event-free survival. PMID: 29128352 the cigarette smoke (CS)-induced loss of SRB1 induced an alteration of sebocytes lipid content, also demonstrated by cholesterol quantification in SRB1 siRNA experiments. In conclusion, exposure to CS, induced SRB1 post-translational modifications in sebocytes and this might affect sebocytes/skin functionality PMID: 27865981 Our analyses revealed no apparent differences in protein expression profiles of SRBs in central and peripheral regions of human donor tissues, indicating that carotenoid-binding proteins rather than transporters are likely to mediate selective accumulation of carotenoids into the macula. PMID: 28947101 Low SRB1 expression is associated with non-alcoholic steatohepatitis but is unchanged in hepatocellular carcinoma. PMID: 28941732 SCARB1 rs5888 and environmental oxidative stress have a prominent role in age-related macular degeneration(ARMD) susceptibility, early ARMD progression to advanced stage disease and even in the outcome of the disease-an area of macular lesion. PMID: 27428740 SCARB1 gene variants are associated with a new lipid phenotype, characterized by high levels of both HDL cholesterol and Lp(a). SCARB1 exonic variants often result in diminished function of translated SR-B1 via reduced binding/intracellular transport of Lp(a). PMID: 27651445 Data suggest that activation of SR-BI by APOAI down-regulates sphingosine 1-phosphate/S1PR2-mediated inflammation in vascular endothelial cells by activating the PI3K/Akt signaling pathway; oxidized-LDL does the opposite. (APOA1 = apolipoprotein A-I; SR-BI/SCARB1 = scavenger receptor class B type I; S1PR2 = sphingosine 1-phosphate receptor 2; PI3K = phosphatidylinositol 3-kinase; Akt = proto-oncogene c-akt) PMID: 28181168 Sustained virologic response was significantly associated with SCARB1 rs10846744 in chronic hepatitis C patients, treated with pegylated interferon-alpha and ribavirin. PMID: 28827115 Model recombinant HDL (rHDL) particles formed in vitro with S1P incorporated into the particle initiated the internalization of S1PR1, whereas rHDL without supplemented S1P did not, suggesting that S1P transported in HDL can selectively activate S1PR1. PMID: 27881715 Data implicate that scavenger receptor class B member 1 (SR-B1) as a target in chronic lymphocytic leukemia (CLL) and high-density lipoproteins nanoparticles (HDL NPs) as targeted monotherapy for CLL. PMID: 28061439 SCARB1 gene polymorphisms may contribute to genetic susceptibility to coronary heart disease. C allele of rs10846744 and the C allele of rs2278986 may serve as risk and protective factors for CHD, respectively. PMID: 28552715 VEGF-A was found to be a prerequisite for the localization of scavenger receptor BI in the plasma membrane of endothelial cells and is a regulatory factor of transendothelial transport of HDL but not LDL. PMID: 28360088 our findings implied that scavenger receptor class B type 1 might serve as a diagnostic and independent prognostic biomarker in clear cell renal cell carcinoma. PMID: 28466781 data support an SR-B1 nibbling mechanism that is similar to that of streptococcal serum opacity factor, which also selectively removes CE and releases apoAI, leaving an apoAII-rich remnant. PMID: 28373285 Using mass spectrometry and site directed mutagenesis, a new Sp1 phosphorylation site Ser702 was defined to be associated with Sp1-HDAC1 interaction and may be important in SR-BI activation, shedding light on the knowledge of delicate mechanism of hepatic HDL receptor SR-BI gene modulation by LDL. PMID: 27320013 down-regulation of SR-BI by endoplasmic reticulum stress in hepatic cells might contribute to the unfavorable effects of metabolic disorders on cholesterol homeostasis and cardiovascular diseases PMID: 27666478 We confirmed associations with papillary thyroid cancer and SNPs in FOXE1/HEMGN, SERPINA5 (rs2069974), FTO (rs8047395), EVPL (rs2071194), TICAM1 (rs8120) and SCARB1 (rs11057820) genes. We found associations with SNPs in FOXE1, SERPINA5, FTO, TICAM1 and HSPA6 and and follicular thyroid cancer PMID: 27207655 We engineered a library of infectious HCV with all codons represented at most positions in the ectodomain of the E2 gene. Nine variants showed reduced dependence on scavenger receptor class B type I (SR-BI) for infection. PMID: 27630236 The data from this ex vivo study suggests that up-regulated SR-B1 protein expression is associated with malignant behaviors of breast cancer and that SR-B1 is an independent predictor for poor survival in breast cancer patients. PMID: 27067809 ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner. PMID: 27377933 As proof-of-concept, human serum exosomes were found to express SR-B1, and HDL NPs can be used to label and isolate them. PMID: 26964503 Shear stress regulates endothelial cell function through SR-B1-eNOS signaling pathway. PMID: 27225585 Data indicate that high scavenger receptor class B type I (SR-BI) expression was an independent unfavorable prognostic factor for overall survival (OS), and that high SR-BI expression could be used as a potential prognostic marker in breast cancer patients. PMID: 26456958 CLA1 transport activity is necessary for the acquisition of host phosphatidylcholine by C. trachomatis. PMID: 26381674 the single nucleotide polymorphism (rs5888) within SCARB1 is independently associated with premature coronary artery disease in a sex-dependent manner. PMID: 26754576 SR-B1 and targeted HDL NPs provide a fundamental advance in studying cholesterol-dependent cellular uptake mechanisms. PMID: 26511855 contributes to LDL transcytosis PMID: 26334034 PGG enhances expression of SR-BI and ABCA1 in J774 and THP-1 macrophages PMID: 26322417 hSR-BII, and to a lesser extent hSR-BI, significantly increase LPS-induced inflammation and contribute to LPS-induced tissue injury in the liver and kidney, two major organs susceptible to LPS toxicity. PMID: 26936883 Intestinal activation of LXR reduces the production of chylomicrons by a mechanism dependent on the apical localization of SR-B1. PMID: 26602218 resistance to HCV in HIV+ patients may be related to genetic variation in SCARB1 or OCLN PMID: 26571379 Nrf2 activation and the expression and oxidative posttranslational modification of SRB1 are impacted in CDKL5 related Rett syndrome. PMID: 26006105 These results suggest that EPA inhibits intestinal beta-carotene absorption by down regulation of SR B1 expression via PPARalpha dependent mechanism and provide an evidence for dietary modulation of intestinal beta-carotene absorption. PMID: 26577021 Data show consistent association of scavenger receptor class B member 1 (SCARB1) variants with high-density lipoprotein cholesterol (HDL-C) across various association analyses, suggesting the role of SCARB1 in lipoprotein-lipid regulatory mechanism. PMID: 26563154 SCARB1 P376L is associated specifically with elevated HDL cholesterol level and risk of coronary heart disease. PMID: 26965621 Propofol up-regulates expression of ABCA1, ABCG1, and SR-B1 through the PPARgamma/LXRalpha pathway in THP-1 macrophage-derived foam cells. PMID: 25600616 SCARB1 missense rs4238001 is statistically significantly associated with incident CHD. PMID: 25993026 Hepatic SR-BI is associated with type 2 diabetes but unrelated to human and murine non-alcoholic fatty liver disease. PMID: 26431876 These observations confirm the role of CD81 in liver-stage malaria and question that of scavenger receptor class B member 1. PMID: 25656410 Variants of SCARB1 and VDR Involved in Complex Genetic Interactions May Be Implicated in the Genetic Susceptibility to Clear Cell Renal Cell Carcinoma. PMID: 25945350

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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