Human RUNX3 (Runt-Related Transcription Factor 3) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-02655P

SDS-PAGE analysis of Human RUNX3 (Runt-Related Transcription Factor 3) - Recombinant Protein, CAT# BLT-02655P, showing >90% purity under 15% SDS-PAGE (Reduced)
Human RUNX3 (Runt-Related Transcription Factor 3) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-02655P
Regular price
$59500
$595.00
Sale price$44500
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Quantity Pricing
Pack Size | Price (USD) |
---|---|
500 µg | $1,030 (Fall Promotion) |
1 mg | $1,870 (Fall Promotion) |
For direct online orders, quantity pricing will be displayed in cart when you add 5x100ug or 10x100ug
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Product Overview
Product Name | Recombinant Human RUNX3 Protein |
Product Overview | This recombinant human RUNX3 protein includes amino acids 53-186aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
Target Uniprot Id | Q13761 |
Recommended Name | Runt-related transcription factor 3 |
Gene Name | RUNX3 |
Synonyms | Runt-related transcription factor 3, AML2, CBFA3, PEBP2aC |
Species | Human |
Predicted Molecular Mass | 17.1 kDa |
Expression System | E.coli |
Expression Range | 53-186aa |
Tag | N-6His |
Purity | >90% |
Formulation | Lyophilized |
Buffer | Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
Storage Condition | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Reconstitution Instruction | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Applications | Positive Control; Immunogen; SDS-PAGE; WB |
Research Area | Epigenetics And Nuclear Signaling |
Target Function | Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. |
Subcellular Location | Nucleus. Cytoplasm. |
Tissue Specificity | Expressed in gastric cancer tissues (at protein level). |