Recombinant Human RheB Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BL-0255NP
Recombinant Human RheB Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BL-0255NP
Catalog No.: BL-0255NP
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | Q15382 |
| Synonym | RheB GTP-Binding Protein Rheb; Ras Homolog Enriched in Brain; RHEB; RHEB2 |
| Background | GTP-Binding Protein Rheb (RHEB) is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. Highest levels of RHEB can be found in the skeletal and cardiac muscle, and it is vital in the regulation of growth and cell cycle progression due to its role in the Insulin/TOR/S6K signaling pathway. RHEB stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling, and it activates the protein kinase activity of mTORC1. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, farnesylation of the protein is required for this activity. |
| Description | Recombinant Human Ras homolog enriched in brain is expressed by our E.coli expression system and the target gene encoding Met1-Met184 is expressed with a GST tag at the N-terminus. |
| Source | E.coli |
| AA Sequence | Met1-Met184 |
| Purity | >95% as determined by reducing SDS-PAGE. |
| Endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Formulation | Recombinant Human RheB Protein was lyophilized from a 0.2 μm filtered solution of 20mM Tris, 10mM GSH, pH 8.0. |
| Reconstitution | Dissolve the lyophilized protein in distilled water. |
| Usage | For Research Use Only |
| Storage | Human RheB protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
