Recombinant Human Ras-Related Protein Rab-2A (RAB2A) Protein (His-SUMO)

Name: Recombinant Human Ras-Related Protein Rab-2A (RAB2A) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-09886P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Ras-Related Protein Rab-2A (RAB2A) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P61019
Target Symbol	RAB2A
Synonyms	9330148M11Rik; C80220; GTP binding protein; LHX; OTTMUSP00000004796; RAB2; RAB2 member RAS oncogene family; RAB2A; RAB2A; member RAS oncogene family; RAB2A_HUMAN; Ras related protein Rab 2A; RAS-associated protein RAB2; Ras-related protein Rab-2A; RP23-183L6.1; Small GTP binding protein RAB2A
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	AYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAATNATHAGNQGGQQAGGGCC
Expression Range	2-212aa
Protein Length	Full Length of Mature Protein
Mol. Weight	39.4kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Required for protein transport from the endoplasmic reticulum to the Golgi complex.
Subcellular Location	Endoplasmic reticulum-Golgi intermediate compartment membrane; Lipid-anchor. Melanosome. Endoplasmic reticulum membrane; Lipid-anchor. Golgi apparatus membrane; Lipid-anchor. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Protein Families	Small GTPase superfamily, Rab family
Database References	HGNC: 9763 OMIM: 179509 KEGG: hsa:5862 STRING: 9606.ENSP00000262646 UniGene: Hs.369017

Gene Functions References

S100A7 affects cell motility and invasion by regulating the RAB2A-associated MAPK signaling cascades. PMID: 27767088
Identify Rab2 as a key factor for autophagic and endocytic cargo delivery to and degradation in lysosomes. PMID: 28483915
our data reveal that RAB2A is new critical membrane trafficking players in the regulation of mesenchymal properties of normal and tumorigenic cells and a critical determinant of an invasive, proteolytic mode of BC progression. PMID: 27255086
RAB2A variance has effect on the structure and function of the PFC and related cognitive functions. PMID: 26249043
This study demonstrated that RAB2A mutation showing the highest statistical significance in Autism and Schizophrenia. PMID: 26938441
The immunohistochemistry-based validation using tissue microarray slides in oral squamous cell carcinoma revealed overexpression of the RAB2A and PRDX1 gene in 80 and 68 % of the tested clinical cases, respectively. PMID: 26159854
Pin1/Rab2A/Erk drives BCSC expansion and tumorigenicity, suggesting potential drug targets. PMID: 25818297
As a result of the translocations, RAB2A-PLAG1, the constitutively active promoter of the partner gene drives the ectopic expression of PLAG1. PMID: 24700772
first X-ray crystal structure of RicA to 2.7 A resolution and have quantified the affinity of RicA binding to human Rab2 in its GDP-bound and nucleotide-free forms PMID: 24251537
The authors identified a specific interaction between the human small GTPase Rab2 and a Brucella spp. protein named RicA. PMID: 21501366
The results of this study provided that RAB2A and SLC38A1, were associated with the density of calbindin-positive neurons and the number of perineuronal oligodendrocytes in psychiatric disorders. PMID: 20308991
Rab2 interacts directly with atypical protein kinase C iota/lambda and inhibits aPKCiota/lambda-dependent GADPH phosphorylation PMID: 14570876
These data demonstrate that Rab2 and Rab6 differentially influence anterograde transport and signaling of GPCRs. PMID: 17716866
ICA69 as a novel Rab2 effector and its role in regulating the early transport of insulin secretory granule proteins PMID: 18187231

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.