Recombinant Human QTRT1 Protein
Beta LifeScience
SKU/CAT #: BL-2040PS
Recombinant Human QTRT1 Protein
Beta LifeScience
SKU/CAT #: BL-2040PS
Collections: Other recombinant proteins, Recombinant proteins
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Product Overview
Tag | N/A |
Host Species | Human |
Synonym | Queuine TRNA-Ribosyltransferase 1, TRNA-GuanineTransglycosylase, TGT, Guanine Insertion Enzyme, EC 2.4.2.29, TGUT, QueuineTRNA-Ribosyltransferase, TGT, Catalytic Subunit, TGT, 43-KD Subunit, FP3235, QueuinetRNA-ribosyltransferase. |
Background | Queuine TRNA-Ribosyltransferase 1 also known as QTRT1 is a member of thequeuine tRNA-ribosyltransferase family. QTRT1 interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. Furthermore, QTRT1 exchangesqueuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), in this manner forming thehypermodified nucleoside queuosine (Q)(7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). |
Description | QTRT1 Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain containing 428a.a. (1-403 a.a) andhaving a molecular weight of 46.7kDa.QTRT1 is fused to a 25a.a. His-tag at N-terminusand purified by unique purification methods. |
Source | E.coli |
AA Sequence | MGSSHHHHHH SSGLVPRGSH MGSEFMAGAA TQASLESAPR IMRLVAECSR SRARAGELWL PHGTVATPVFMPVGTQATMK GITTEQLDAL GCRICLGNTY HLGLRPGPEL IQKANGLHGF MNWPHNLLTD SGGFQMVSLVSLSEVTEEGV RFRSPYDGNE TLLSPEKSVQ IQNALGSDII MQLDDVVSST VTGPRVEEAM YRSIRWLDRCIAAHQRPDKQ NLFAIIQGGL DADLRATCLE EMTKRDVPGF AIGGLSGGES KSQFWRMVAL STSRLPKDKP RYLMGVGYATDLVVCVALGC DMFDCVFPTR TARFGSALVP TGNLQLRKKV FEKDFGPIDP ECTCPTCQKH SRAFLHALLHSDNTAALHHL TVHNIAYQLQ LMSAVRTSIV EKRFPDFVRD FMGAMYGDPT LCPTWATDAL ASVGITLG. |
Purity | >80.0% as determined by SDS-PAGE. |
Endotoxin | <1.0 EU per μg by the LAL method. |
Formulation | QTRT1 protein solution (0.25mg/ml) containing Phosphatebuffered saline (pH7.4), 30% glycerol and 1mM DTT. |
Stability | Recombinant protein is stable for 12 months at -70°C |
Usage | For Research Use Only |
Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |