Recombinant Human PTPRB Protein

Name: Recombinant Human PTPRB Protein
Brand: Beta LifeScience
SKU: BL-0027SG
Availability: InStock

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	NM_002837
Synonym	PTPB, HPTPB; VEPTP; HPTP-BETA; R-PTP-BETA
Background	PTPRB contains multiple repeated fibronectin type-lll domains extracellularly, a single transmembrane segment and only one protein tyrosine phosphatase (PTP) domain intracellularly, and thus belongs to receptor type PTP family (1). PTPRB negatively regulates angiogenesis and blood vessel remodeling via interacting with vascular growth factor tyrosine kinases such as TIE2, CDH5, and KDR (2). PTPRB has been reported to be one of the recurrent mutations in angiosarcoma tumors (3).
Description	Recombinant human PTPRB (1552-end) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source	Sf9 insect cells
AA Sequence	1552a.a.-end
Molecular Weight	116 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Active
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human PTPRB Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Protein-tyrosine phosphatase family, Receptor class 3 subfamily
Database References	HGNC: 9665 OMIM: 176882 KEGG: hsa:5787 STRING: 9606.ENSP00000334928 UniGene: PMID: 27314562 STIM1-induced Ca(2+) signaling activates Pyk2 to inhibit the interaction of VE-PTP and VE-cadherin and hence increase endothelial permeability. PMID: 28385807 Results provide evidence that PTPRB and PLCG1 mutations are driving events in a subset of secondary angiosarcomas. PMID: 24795022 VE-PTP activates TIE2 and stabilizes retinal and choroidal blood vessels PMID: 25180601 these results suggest that the polarized redistribution of VE-PTP in response to shear stress plays an important role in the regulation of endothelial cells function by blood flow. PMID: 24451369 The endothelial phosphatase PTPRB, a negative regulator of vascular growth factor tyrosine kinases, harbored predominantly truncating mutations in 10 of 39 angiosarcoma tumors. PMID: 24633157 zinc(II) ions regulate receptor protein-tyrosine phosphatase beta activity at picomolar concentrations. PMID: 22275360 vascular endothelial protein tyrosine phosphatase contributes to endothelial morphogenesis. Silencing of VE-PTP expression was accompanied by increased VEGF receptor-2 tyrosine phosphorylation and activation of downstream signaling pathways. PMID: 19136612 Suggest that VE-PTP, in cooperation with integrins, regulates the spreading and migration of endothelial cells during angiogenesis. PMID: 20301196 The PTP1B has been observed over expressed in human breast cancer patients, suggesting its role in cell proliferation. PMID: 15638728 the Ser127Gly polymorphism in PTPRB is associated with substance abuse vulnerability in three independent case-control samples, but was not associated with alcoholism in Japanese subjects PMID: 18361428 These results reveal a novel role for HPTPbeta in modulating Ang-1-Tie2 signaling and endothelial cell survival. PMID: 19116766

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.