Recombinant Human PTK9L) Protein

Name: Recombinant Human PTK9L) Protein
Brand: Beta LifeScience
SKU: BL-1407SG
Availability: InStock

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	BC003161
Synonym	TWF2, A6r, A6RP, MSTP011
Background	Protein tyrosine kinase 9-like (A6-related protein) belongs to the actin-binding proteins ADF family, the Twinfilin subfamily. It was initially cloned by interaction using the catalytic domain of protein kinase Czeta (PKCzeta) as bait. It contains actin-binding sites and ATP-binding sites. Involved in motile and morphological processes by regulating actin polymerization.
Description	Full-length recombinant human PTK9L (A6R) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source	Sf9 insect cells
AA Sequence	Full Length
Molecular Weight	65 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human PTK9L) Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia.
Subcellular Location	Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell projection, stereocilium. Note=Perinuclear and G-actin-rich cortical actin structure sublocalization.
Protein Families	Actin-binding proteins ADF family, Twinfilin subfamily
Database References	HGNC: 9621 OMIM: 607433 KEGG: hsa:11344 STRING: 9606.ENSP00000303908 UniGene: Hs.436439
Tissue Specificity	Ubiquitously expressed (at protein level).

Gene Functions References

twinfilin-2 is a protein that is involved in neurite outgrowth PMID: 17910947

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.