Recombinant Human Probable Histidine--Trna Ligase, Mitochondrial (HARS2) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09998P

Greater than 90% as determined by SDS-PAGE.
Recombinant Human Probable Histidine--Trna Ligase, Mitochondrial (HARS2) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09998P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human Probable Histidine--Trna Ligase, Mitochondrial (HARS2) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | P49590 |
Target Symbol | HARS2 |
Synonyms | 4631412B19Rik; AI593507; HARS related; Hars2; HARSL; HARSR; HisRS; Histidine translase; Histidine tRNA ligase; Histidine tRNA ligase homolog; Histidine tRNA ligase like; Histidine--tRNA ligase; Histidine--tRNA ligase-like; Histidyl tRNA synthetase 2; Histidyl tRNA synthetase 2 mitochondrial; Histidyl tRNA synthetase like; HO3; mitochondrial; Probable histidyl tRNA synthetase mitochondrial; Probable histidyl-tRNA synthetase; SYHM_HUMAN |
Species | Homo sapiens (Human) |
Expression System | E.coli |
Tag | N-6His-SUMO |
Target Protein Sequence | QSQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES |
Expression Range | 34-506aa |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 69.4kDa |
Research Area | Metabolism |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Mitochondrial aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). |
Subcellular Location | Mitochondrion. |
Protein Families | Class-II aminoacyl-tRNA synthetase family |
Database References | |
Associated Diseases | Perrault syndrome 2 (PRLTS2) |
Tissue Specificity | A high level expression is seen in the heart, kidney and skeletal muscle while a lower level expression is seen in the brain and liver. |
Gene Functions References
- Mutations in mitochondrial histidyl tRNA synthetase HARS2 cause ovarian dysgenesis and sensorineural hearing loss of Perrault syndrome. PMID: 21464306
- This protein has been found differentially expressed in the anterior cingulate cortex in men patients with schizophrenia. PMID: 20381070